摘要
目的:将富含血小板血浆(PRP)与纳米羟基磷灰石(nHA)/胶原(Co)复合,通过动物实验评价复合材料的生物学性能,充分发挥每种材料的优点,使之成为组织工程学中一种新型替代骨组织的生物活性材料和支架材料。方法:将nHA和胶原复合并将其制备成nHA/Co复合膜后加入PRP制备成PRP/nHA/Co复合材料。实验动物制备大小为5mm×5mm的牙周缺损,右侧为实验组,在相应牙位置入PRP/nHA/Co复合膜材料;左侧对照组不放置任何材料。术后第4、8周行64排CT片和组织病理检查,观察成骨效果。结果:动物生存状态良好,牙龈附着愈合良好。CT观察实验组出现新生牙槽骨,骨嵴基本恢复到原来高度;对照组牙槽嵴高度未见增加。HE染色观察对照组缺损处周围见大量炎性细胞,纤维组织增生,无软骨形成并有大量上皮长入;实验组可见大量新生的牙槽骨。结论:PRP/nHA/Co复合生物膜可以作为组织工程学中一种新型替代骨组织的生物活性材料和支架材料。
Objective： To composite PRP, nHA and collagen together, and evaluate the bio-characteristics of this compound material. In this way, we want to take advantage of every one to make it a new substitute for bone regeneration. Methods： Put nHA and Co together to prepare nHA/Co compound membrane, then add PRP to prepare PRP/nHA/Co compound material. Animals were prepared periodontal bone defects of about 5 × 5mm, The right side of the experimental group placed the PRP / nHA / Co compound material;the left side of the control group did not place any material. 4,8 weeks after implantation, the effectiveness of bone formation was evaluated by means of 64-slice CT observation and histological examination. Results： The animals grow healthy, with the gingival healing well. CT observation：new alveolar bone formation and the ridge height growed to normal. While, the ridge height was not growed in the control group. Tissue observation：Foreign-body granuloma could be seen in the absence area, a lot of lymphocyte cells and no bone tissue, the junctional epithelium grow fast to form long junctional epithelium in the control group; A lot of new bone could he seen in the Experimental Group. Conclusion： PRP/nHA/Co compound membrane can be used as a new bio-active material and vector material as bone substitution in tissue engineering.
出处
《口腔医学研究》
CAS
CSCD
2012年第2期110-113,共4页
Journal of Oral Science Research
基金
吉林省科技厅社会发展处重点课题(编号:20070426-1)
