中国汉族儿童CYP3A5*3基因型与急性淋巴细胞白血病易感性的关联研究(英文)

Association of CYP3A5*3 with Acute Lymphoblastic Leukemia susceptiveness in Chinese Han children

目的:在中国汉族儿童中探讨白血病易感性与CYP3A5*3的频率之间的相关性。方法:共收集172名中国汉族儿童血液,包括52名急性白血病患者(27女性,25男性)和120名健康儿童(53女性,67男性)。提取DNA,然后用PCR-RFLP方法检测基因型。结果:CYP3A5*3在人群中的分布符合Hardy-Weinberg平衡。儿童白血病中CYP3A5*3频率为0.721(95%CI:0.549,0.893),而健康儿童中为0.758(95%CI:0.674,0.842),两者之间无明显差异,并且也无男女性别差异。结论:CYP3A5*3是健康儿童和白血病患者中的常见基因型,健康儿童和白血病患儿中CYP3A5*3频率一致,因此CYP3A5*3与儿童白血病易感性之间无关联。

ABSTRACT To investigate the association of CYP3A5 ＊ 3 with Acute Lymphoblastic Leukemia （ALL） susceptiveness in Chinese Han children. METHODS： CYP3A5 genotype was analyzed from 172 Chinese han children, including 52 ALL patients（27 female and 25 male） and 120 healthy controls（80 female and 92 male）. Genomic DNA was extracted from blood sample, and PCR-RFLP was used to identify the polymorphism. RESULTS： This polymorphism were matched to HardyWeinberg equilibrium in all group. The allelic frequency of CYP3A5＊ 3 was 0. 721 （950//00 CI： 0. 549, 0. 893） in patients while 0.758 （95% CI： 0.674, 0.842） in healthy controis. There was no significant difference existed in CYP3A5＊ 3 frequencies between patients and controls （P=0. 275）, males and females （P= 0. 432） in the total population. CONCLUSION： The CYP3A5＊ 3 variant represents a common polymorphism in Chinese healthy children andALL, and this polymorphism is poor association with ALL susceptiveness in children.