酵母双杂交中诱饵蛋白载体的构建与鉴定方法

Methods of Construction and Identification of the Bait Vector in Yeast Two-Hybrid System

酵母双杂交已是研究蛋白质相互作用的经典方法之一。该方法以真核生物酵母为模型,在体内研究活细胞内蛋白质的相互作用,具有高度敏感性,被很多研究者采用。综述了酵母双杂交系统中诱饵蛋白载体的构建,及其在转化酵母中的毒性、自激活性检测研究的最新进展。

The yeast two-hybrid system was one of conventional methods to probe of the protein-protein interactions. This model which was first developed by Fields and Song in 1998, explored the interactions between cellular proteins in vivo. Many researchers favoured this method in the study of protein-protein and protein-DNA interactions because of its high sensitivity. The development of the construction of the bait protein vector in yeast two-hybrid system, its self-activation and toxic to the host yeast were summarized.