摘要
目的:探讨急性心肌梗死前、再灌注前及再灌注时等不同时期,无创性肢体缺血预适应在减轻大鼠缺血/再灌注损伤中的作用。方法:所有大鼠随机分为4组(每组10只):心肌缺血/再灌注损伤组(A组)、急性心肌梗死前肢体缺血预适应组(B组)、再灌注前肢体缺血预适应组(C组)和再灌注初期肢体缺血预适应组(D组)。观察各组心电、心肌缺血范围(AAR)、心肌梗死范围(IA)、心肌梗死部位质量与左心室质量(LV)的比值(IA/LV)、梗死范围与缺血范围的比值(IA/AAR)、CK-MB值。结果:与A组相比,B、C、D 3组ST段抬高幅度、CK-MB值、IA/LV和IA/AAR比值均显著降低(P<0.01)。结论:无创性肢体缺血预适应在急性心肌梗死前、再灌注前及再灌注时的各个时期应用,都能明显降低大鼠心肌缺血/再灌注时的ST段抬高幅度,明显降低心肌酶水平,明显缩小心肌坏死面积。
Objective:To observe the protective effects of noninvasive limb ischemic conditioning on the myocardial ischemia/reperfusion(I/R) injury in different time windows of acute myocardial infarction(AMI) rats.Methods:All wistar rats were randomly divided into four groups(n=10 in each group):(1)Myocardial ischemia /reperfusion group(group A),limb ischemic preconditioning before AMI group(group B),limb ischemic preconditioning before reperfusion group(group C),limb ischemic preconditioning during reperfusion group(group D).We recorded the ST-segment during AMI and reperfusion,and measured myocardial area at risk(AAR),myocardial infarcted area(IA),IA/LV,IA/AAR,MB isoenzyme of creatine kinase(CK-MB) after 3 h of reperfusion.Result:The elevation of ST-segment is decreased,and IA,IA/AAR,CK-MB are significantly less in group B,C and D as compared to group A(P〈0.01).Conclusion:The noninvasive limb ischemic conditioning in different time windows of AMI rats can prevent myocardial ischemia-reperfusion injury.
出处
《心肺血管病杂志》
CAS
2012年第1期76-78,共3页
Journal of Cardiovascular and Pulmonary Diseases
基金
黑龙江省教育厅科技研究项目(编号:10543023)
关键词
心肌梗死
缺血/再灌注损伤
缺血预适应
远程缺血预适应
Myocardial infarction
Ischemia/Reperfusion injury
Ischemic preconditioning
Remote ischemic preconditioning