不同时期无创肢体缺血预适应对急性心肌梗死大鼠的保护作用

Protection of noninvasive limb ischemic preconditioning in different time windows of acute myocardial infarction Rats

目的:探讨急性心肌梗死前、再灌注前及再灌注时等不同时期,无创性肢体缺血预适应在减轻大鼠缺血/再灌注损伤中的作用。方法:所有大鼠随机分为4组(每组10只):心肌缺血/再灌注损伤组(A组)、急性心肌梗死前肢体缺血预适应组(B组)、再灌注前肢体缺血预适应组(C组)和再灌注初期肢体缺血预适应组(D组)。观察各组心电、心肌缺血范围(AAR)、心肌梗死范围(IA)、心肌梗死部位质量与左心室质量(LV)的比值(IA/LV)、梗死范围与缺血范围的比值(IA/AAR)、CK-MB值。结果:与A组相比,B、C、D 3组ST段抬高幅度、CK-MB值、IA/LV和IA/AAR比值均显著降低(P<0.01)。结论:无创性肢体缺血预适应在急性心肌梗死前、再灌注前及再灌注时的各个时期应用,都能明显降低大鼠心肌缺血/再灌注时的ST段抬高幅度,明显降低心肌酶水平,明显缩小心肌坏死面积。

Objective：To observe the protective effects of noninvasive limb ischemic conditioning on the myocardial ischemia/reperfusion（I/R） injury in different time windows of acute myocardial infarction（AMI） rats.Methods：All wistar rats were randomly divided into four groups（n=10 in each group）：（1）Myocardial ischemia /reperfusion group（group A）,limb ischemic preconditioning before AMI group（group B）,limb ischemic preconditioning before reperfusion group（group C）,limb ischemic preconditioning during reperfusion group（group D）.We recorded the ST-segment during AMI and reperfusion,and measured myocardial area at risk（AAR）,myocardial infarcted area（IA）,IA/LV,IA/AAR,MB isoenzyme of creatine kinase（CK-MB） after 3 h of reperfusion.Result：The elevation of ST-segment is decreased,and IA,IA/AAR,CK-MB are significantly less in group B,C and D as compared to group A（P〈0.01）.Conclusion：The noninvasive limb ischemic conditioning in different time windows of AMI rats can prevent myocardial ischemia-reperfusion injury.