摘要
MicroRNAs （miRNAs） are small, noncoding RNAs which can often act as an oncogene or a tumor suppressor. Several miRNAs are associated with the development of hepatoceUular carcinoma （HCC）. We demonstrated that miR-125b significantly suppresses HCC cell proliferation and promotes apoptosis by inhibiting the gene expression of the anti-apoptotic protein, Bcl-2. Bioinformatic analysis indicated that the 3tUTR of Bcl-2 has binding sites for miR-I25b, Luciferase reporter assay confirmed the ability of miR-125b to dramatically suppress Bcl-2 transcription, suggesting that Bcl-2 is a target gene for miR-125b. We concluded that miR-125b acts as a tumor suppressor in hepatic tumor development by targeting Bcl-2 and inducing cancer celt apoptosis.
MicroRNAs （miRNAs） are small, noncoding RNAs which can often act as an oncogene or a tumor suppressor. Several miRNAs are associated with the development of hepatoceUular carcinoma （HCC）. We demonstrated that miR-125b significantly suppresses HCC cell proliferation and promotes apoptosis by inhibiting the gene expression of the anti-apoptotic protein, Bcl-2. Bioinformatic analysis indicated that the 3tUTR of Bcl-2 has binding sites for miR-I25b, Luciferase reporter assay confirmed the ability of miR-125b to dramatically suppress Bcl-2 transcription, suggesting that Bcl-2 is a target gene for miR-125b. We concluded that miR-125b acts as a tumor suppressor in hepatic tumor development by targeting Bcl-2 and inducing cancer celt apoptosis.
基金
supported by the National High Technology Research and Development Program of China(No. 2006AA02A107)
the Major State Basic Research Program of China(Nos.2009CB521704 and 2010CB945504)
the National Nature Science Foundation of China(No.30873031)
