人基因BAG-1高表达载体的构建及其对肺腺癌细胞放射敏感度的影响

Effect of Expression Vector of Human BAG-1 Gene on Radio-sensitivity of Lung Adenocarcinoma Cells

目的通过构建BAG-1高表达载体来研究BAG-1在肺腺癌细胞放射敏感度中的作用。方法提取人肺癌A549细胞中总RNA,通过RT-PCR扩增BAG-1基因,构建pcDNA3.1-BAG-1表达载体,转染肺癌细胞A549,通过Western blot筛选出BAG-1高表达A549细胞株。通过克隆形成实验,研究BAG-1对肺腺癌细胞放射敏感度的影响。结果成功构建pcDNA3.1-BAG-1载体;筛选出BAG-1高表达A549细胞株;BAG-1高表达降低肺腺癌细胞放射敏感度。结论 BAG-1高表达参与放射抵抗,有望成为肿瘤治疗的新靶点。

Objective To explore the effect of BAG-1 on lung adenocarcinoma cells radio-sensitivity through constructing high expression vector of BAG-1.Methods Total RNA was extracted from A549 lung cancer cell lines.BAG-1 gene was amplified by RT-PCR.The expression vector pcDNA3.1-BAG-1 was constructed,and then transfected into lung cancer cells A549.A549 cell lines with high expression of BAG-1 was selected by Western blot.Effect of BAG-1 on lung cancer cell radio-sensitivity was investigated by cloning experiments.Results The high expression vector of pcDNA3.1-BAG-1 was constructed successfully.High expression of BAG-1 decreased lung adenocarcinoma cells radio-sensitivity.ConclusionHigh expression of BAG-1 was related to radiation resistance and may be a novel target for cancer therapy.