血管生成素-2及其受体在卵巢癌组织中的表达及与血管生成的关系

Relationship between Expressions of Angiopoietin-2 and Its Receptors and Angiogenesis in Ovarian Cancer

目的探讨血管生成素-2(Ang-2)及其受体(Tie-2)在卵巢癌中的表达及与血管生成、临床病理特征的关系。方法采用RT-PCR法检测25例正常卵巢组织标本、25例良性卵巢肿瘤和65例恶性卵巢肿瘤中Ang-2及其受体Tie-2的表达水平,并采用免疫组织化学法检测各标本的微血管密度(MVD)。结果恶性组MVD显著高于良性组(t=2.61,P<0.05),不同临床分期的卵巢癌MVD的差异有统计学意义(F=4.618,P<0.05)。恶性组Ang-2 mRNA阳性率和表达水平显著高于良性组和对照组(P<0.05),不同临床分期的卵巢癌标本Ang-2 mRNA表达水平有显著性差异(0.298±0.022 vs.0.206±0.086,P<0.05)。恶性组MVD与其Ang-2 mRNA表达水平呈正相关关系(rMV=0.685,P<0.05)。仅在5例恶性卵巢癌标本中检测到Tie-2 mRNA表达,良性组和对照组未检出。结论恶性卵巢癌组织中高表达Ang-2,其与卵巢癌肿瘤血管大量形成有关。

Objective To explore the correlations between the expressions of Angiopoietin-2（Ang-2） and its receptor（Tie-2） and the microvessel density（MVD）,as well as several clinicopathologic parameters in ovarian carcinomas.Methods The expressions of Ang-2 and Tie-2 were detected By RT-PCR,and MVD by the immunohistochemical mothed in 25 specimens of normal ovaries,25 specimens of benign ovarian tumors and 65 specimens of ovarian cancer.Results The MVD in the ovarian cancer group was significantly higher than that in the benign ovarian tumors group（t=2.61,P0.05）,moreover there was a significantly varied MVD among different clinical stages of ovarian cancer（F=4.618,P0.05）.Both positive rate and level of Ang-2 mRNA were markedly highest in the ovarian cancer group than those in the benign ovarian tumors group and the control group（P0.05）.There was a significant difference of Ang-2 mRNA in different clinical stages of ovarian cancer（0.298±0.022 vs.0.206±0.086,P0.05）.A positive correlation was found between the MVD and the expression level of the Ang-2 mRNA（rMV=0.685,P0.05）.Tie-2 mRNA was only observed from 5 specimens of ovarian cancer.Conclusion Ang-2 was high-expression.and associated with the increased formation of tumor blood vessels in ovarian cancer.