牛膝多肽对局灶性脑缺血大鼠坐骨神经功能指数的影响

The effects of A.bidentata polypeptides on sciatic function index in focal cerebral ischemic rats

目的:观察牛膝多肽对局灶性脑缺血后大鼠坐骨神经功能损伤的影响,为其应用于临床提供实验依据。方法:制备局灶性脑缺血-大脑中动脉阻塞模型,血管阻塞再通后连续6 d进行尾静脉注射牛膝多肽。术后7、30 d进行足迹实验,观察牛膝多肽对局灶性脑缺血后坐骨神经功能下降的影响。结果:于大脑中动脉阻塞后7、30 d,SD大鼠坐骨神经功能指数的百分率明显增加(P<0.01)。尾静脉注射不同剂量的牛膝多肽后,坐骨神经功能指数百分率均有不同程度的下降,其中牛膝多肽中剂量组(ABPP(0.2 mg/kg)),无论是大脑中动脉阻塞后7 d还是30 d,其坐骨神经功能指数百分率均较生理盐水组明显下降(P<0.01)。结论:牛膝多肽对大脑中动脉阻塞引起的坐骨神经功能下降具有保护作用。

Objective： To observe the effects of A.bidentata polypeptides（ABPP） on the sciatic function in focal cerebral ischemic rats,and provide the experimental evidence for its clinical applications.Methods： Middle cerebral artery occlusion（MCAO） was employed to establish in rats,then ABPP was injected daily via the tail vein for 6 days.After 7 days and 30 days,the sciatic nerve function index was measured to observe the neuroprotective effect of ABPP.Results： 7 days and 30 days after MCAO,the percentage of sciatic nerve function index of rats increased significantly（P0.01）.However,ABPP,especially in dose of 0.2 mg/kg,could inhibit the increased percentage of sciatic nerve function index induced by MCAO in rats（P0.01）.Conclusion： ABPP have neuroprotective effect on sciatic nerve dysfunction induced by MCAO.