摘要
目的:研究戊巴比妥钠在裸鼠皮下-肝间接原位移植人肝细胞肝癌模型建立中的合适剂量。方法:共30例接种肝癌细胞株的皮下瘤雄性裸鼠取瘤后,对共150只雄性裸鼠行皮下-肝包膜下瘤体原位移植,观察供受体麻醉过程、药物剂量和效果。结果:供体手术戊巴比妥钠麻醉的剂量以80 mg/kg合适,受体手术戊巴比妥钠麻醉剂量以70 mg/kg合适。结论:在裸鼠皮下-肝脏原位肝癌模型建立的手术中,戊巴比妥钠是一种理想的麻醉药品。
Objective: To study proper dosage of pentobarbital sodium in establishing human hepatoma model from substance to orthotropic transplantation with hepatocellular carcinoma cell lines in nude mice.Methods: 150 male nude mice models from substance to orthotropic transplantation was established by with 30 male nude mice sub-skin transplanted models with hepatocellular carcinoma cell lines.The transplantation was performed by using pentobarbital sodium,and the dosage effect relationship was observed in the anesthesia procedure.Results: Pentobarbital sodium dosage of donor was 80 mg/kg of choice and that of recipient was 70 mg/kg.Conclusion: Pentobarbital sodium is a perfect anesthetic used in establishing human hepatoma model from substance to orthotropic transplantation.
出处
《南通大学学报(医学版)》
2012年第1期13-15,共3页
Journal of Nantong University(Medical sciences)
基金
国家自然科学基金青年基金资助项目(81000984)
江苏省高校自然科学研究项目(10KJB320014)
南通市社会发展科技计划(S2010019)
关键词
肝癌模型
移植
麻醉
戊巴比妥钠
裸鼠
hepatoma model
transplantation
anesthesia
pentobarbital sodium
nude mouse