肝癌中肝细胞生长因子受体的表达及意义

The study for the expression of hepatocyte growth factor receptor in the serum and tissues in patients with hepatic carcinoma

目的:研究肝细胞生长因子受体(c-Met)在肝癌患者组织及血浆中的表达,探讨c-Met在肝癌发生、发展所起的作用。方法:对肝癌及慢性肝炎患者进行调查,并且设立正常对照组,应用酶联免疫吸附法检测各组血浆c-Met水平;应用半定量RT-PCR法和免疫组织化学法分别检测各组肝组织中的c-Met mRNA及c-Met蛋白的表达。结果:肝癌患者血浆中的c-Met水平明显高于慢性肝炎患者和正常人群(均P<0.05)。肝癌组织中的c-Met水平明显高于慢性肝炎组织和正常肝组织(均P<0.05)。结论:c-Met在肝癌组织及血浆中呈高表达,可能与肝癌的发生、发展有关。

Objective： To study the expression of hepatocyte growth factor receptor（c-Met） in the serum and tissues in patients with hepatic carcinoma,explore the role of c-Met in the tumorigenesis and progression of human hepatic carcinoma.Methods： Study the patients with hepatic carcinoma or hepatitis,and establish normal control group,measure the plasma c-Met concentrations in them by enzyme-linked immunosorbent assay（ELISA）.The expression of c-Met mRNA and protein were detected in liver tissue of them by using semi-quantitative reverse transcription-polymerase chain reaction（RT-PCR） and immunohistochemistry respectively.Results： The plasma c-Met concentrations in patients with hepatic carcinoma was higher than those in hepatitis and normal control group（P0.05）.The expression level of c-Met in hepatic carcinoma was higher than those in hepatitis and normal hepatic tissue（P0.05）.Conclusion： The expression of c-Met in the serum and tissues in pationts with hepatic carcinoma was high,c-Met may be involved in the tumorigenesis and progression of hepatic carcinoma.