艾灸预处理对大鼠应激性胃黏膜损伤增殖修复相关因子的影响

Effect of moxibustion preconditioning on proliferation and repair factors in gastric mucosa of rats with stressinduced gastric mucosal lesions

目的:观察艾灸预处理对应激性胃黏膜损伤大鼠血清和胃黏膜表皮生长因子(EGF)、转化生长因子-α(TGF-α)、胃黏膜三叶因子家族-1(TFF1)、增殖细胞核抗原(PCNA)的影响,探讨艾灸预处理促进胃黏膜损伤增殖修复的作用机制.方法:将48只健康SD大鼠随机分为4组,即空白组、模型组、艾灸穴位组、艾灸非穴组.束缚冷应激法制作大鼠应激性胃黏膜损伤模型.造模之前,艾灸组选取足三里、中脘、脾俞和胃俞等穴位行艾灸预处理8d,艾灸非穴组选取非穴对照点进行预处理.以Guth法计算胃黏膜损伤指数,光镜下观察大鼠胃黏膜组织学改变,放射免疫法测定血清EGF与TGF-α的含量,酶免法检测胃黏膜组织中EGF、TGF-α、TFF1和PCNA的含量.结果:与模型组和艾灸非穴组比较,艾灸足三里、中脘等穴位可使应激性胃黏膜损伤大鼠胃黏膜损伤指数明显下降(14.667±5.710vs27.250±7.448,24.750±7.300,P<0.01),血清EGF、TGF-α含量升高(2.167±0.756vs1.147±0.983,1.358±0.962,P<0.05;11.170±1.315vs4.585±0.720vs5.118±0.659,P<0.01),胃黏膜EGF、TGF-α和PCNA含量升高(343.560±27.644vs269.610±45.119,279.590±58.890,P<0.05;147.470±17.784vs115.530±24.319,116.620±14.908,P<0.01;191.910±37.262vs154.580±18.910,152.450±20.333,P<0.05);与模型组比较,艾灸穴位组胃黏膜TFF1含量明显升高(4.573±0.121vs3.654±0.507,P<0.05).结论:艾灸足三里、中脘等穴位预处理可减轻束缚水浸应激所造成大鼠胃黏膜损伤、促进胃黏膜损伤组织增殖修复,可能是通过上调胃黏膜损伤增殖修复相关因子(EGF、TGF-α、TFF1和PCNA)而达到其促胃黏膜损伤修复的作用.

AIM： To observe the effect of moxibustion pre- conditioning on proliferation and repair factors （EGF, TGF-α, TFF1 and PCNA） in the serum and gastric mucosa of rats with stress-induced gas- tric mucosal lesions, and to explore the possible mechanisms involved. METHODS： Forty eight SPF-class SD rats were randomly divided into blank group, model group, acupoint moxibustion （AM） group and non-acupoint moxibustion （N-AM） group. After 8 days of pretreatment, the rats except those in the blank group received water restraint stress （WRS） to induce gastric mucosal lesions. The gastric mucosal injury index was evaluated us- ing the Guth＇s method. Histological changes in the gastric mucosa were observed by light microscopy. Serum contents of EGF and TGF-α were measured by radioimmunoassay and the contents of EGF, TGF-α, TFF1 and PCNA in the gastric mucosa were detected by enzyme-linked immunosorbent assay （ELISA）. RESULTS： Compared to the model group and N-AM group, gastric mucosal injury index de- creased markedly （14.667 ± 5.710 vs 27.250 ± 7.448, 24.750 ± 7.300, both P 〈 0.01） while the contents of EGF and TGF-α in the serum （2.167 ± 0.756 vs 1.147 ± 0.983, 1.358 ± 0.962, both P 〈 0.05; 11.170 ± 1.315 vs 4.585 ± 0.720 vs 5.118 ± 0.659, both P 〈 0.01） and those of EGF, TGF-α and PCNA in the gastric mucosa （343.560 ± 27.644 vs 269.610 ± 45.119, 279.590 ± 58.890, both P 〈 0.05; 147.470 ± 17.784 vs 115.530 ± 24.319, 116.620 ± 14.908, both P 〈 0.01; 191.910 ± 37.262 vs 154.580 ± 18.910, 152.450 ± 20.333, both P 〈 0.05） increased significantly in the AM group. Com- pared to model group, the content of TFF1 in the gastric mucosa increased significantly （4.573 ± 0.121 vs 3.654 ± 0.507, P 〈 0.05） in the AM group. CONCLUSION： Moxibustion preconditioningat acupoints can repair stress-inducd gastric mucosal injury and promote gastric mucosa cell proliferation via mechanisms which are closely related to elevating the contents of factors for proliferation and repair （EGF, TGF-α, TFF1 and PCNA） in the serum and gastric mucosa.