复合羟丁基壳聚糖纳米载体携载CD133+抗体温敏膜对猪髂动脉内皮细胞生长的影响

Influence of hydroxybutyl-chitosan nanoparticles carries CD133+anti-thermosensitive membrane on growth of endothelial cells of pig iliac artery

目的:观察复合羟丁基壳聚糖纳米载体携载CD133+抗体温敏膜对培养的猪髂动脉内皮细胞生长的影响。方法:将猪髂动脉内皮细胞接种于裸钴铬合金片膜(裸金属支架组)、雷帕霉素药物涂层钴铬合金片膜(雷帕霉素支架组)、复合羟丁基壳聚糖纳米载体携载CD133+抗体温敏膜钴铬合金片膜(抗温敏膜组),培养24h。在光镜下观察细胞形态,以活细胞计数器(CCK-8)测定细胞增殖;以酶联免疫吸附试验(ELISA)试剂盒测定各组血管内皮生长因子(VEGF)、血小板衍化生长因子(PDGF)的水平。结果:动脉内皮细胞在抗温敏膜上生长良好,形态正常。培养24h,与裸金属支架组、雷帕霉素支架组比较,在抗温敏膜上存活的内皮细胞[光密度值:(0.812±0.003)、(0.783±0.005)比(0.903±0.003)],VEGF水平[(11.583±1.281)ng/L、(9.145±1.065)ng/L比(19.731±2.773)ng/L]显著升高,PDGF水平显著降低[(113.627±17.369)ng/μl、(91.251±17.097)ng/μl比(66.278±18.045)ng/μl],P均<0.05。结论:复合羟丁基壳聚糖纳米载体携载CD133+抗体温敏膜有助于血管内皮细胞的增殖。

Objective：To observe influence of influence of hydroxybutyl-chitosan nanoparticles carries CD133＋anti-thermosensitive membrane on growth of endothelial cells of pig iliac artery.Methods：Endothelial cells of pig iliac artery were cultured on bare cobalt-chromium alloy film（bare metal stent group）,rapamycin-eluting cobalt-chromium alloy film（rapamycin stent group） and influence of hydroxybutyl-chitosan nanoparticles carries CD133＋anti-thermosensitive membrane cobalt-chromium alloy film（anti-thermosensitive membrane group） respectively for 24h.Cell forms were observed under light microscope and cell proliferation was measured by cell counting kit-8（CCK-8）.Enzyme linked immunosorbent assay（ELISA） was used to measure levels of vascular endothelial growth factor（VEGF） and platelet derived growth factor（PDGF） in all groups.Results：Arterial endothelial cells grew well on anti-thermosensitive membrane and cell forms were normal.Compared with bare metal stent group and rapamycin stent group after 24h-culturing,there were significant increase in number of endothelial cells [optical density：（0.812±0.003）,（0.783±0.005） vs.（0.903±0.003）]and VEGF level [（11.583±1.281） ng/L,（9.145±1.065） ng/L vs.（19.731±2.773） ng/L],and significant decrease in PDGF level [（113.627±17.369） ng/μl,（91.251±17.097） ng/μl vs.（66.278±18.045） ng/μl]in anti-thermosensitive membrane group,P〈0.05 all.Conclusion：Influence of hydroxybutyl-chitosan nanoparticles carries CD133＋anti-thermosensitive membrane can improve proliferation of vascular endothelial cells.