原发性高血压患者血清CD40水平及脉搏波速率与CD40基因多态性的关系

Gene polymorphism of CD40 related with serum level of CD40 and pulse wave velocity in patients with essential hypertension

目的:探讨原发性高血压患者CD40基因多态性与血清CD40水平及脉搏波速率的联系。方法:研究对象为432名原发性高血压患者。以酶联免疫反应法测定血清可溶性CD40(sCD40)水平。用Taqman聚合酶链式反应(PCR)方法测定CD40基因三种类型(A/G、C/T、T/C),分析CD40基因多态性与血清CD40浓度、脉搏波速率(PWV)的联系。结果:有CD40基因A/G中的GA[GA:(43.4±4.2)pg/ml比AA:(29.6±1.6)pg/ml,GG:(39.8±6.5)pg/ml]、C/T中的CT[CT:(42.1±4.2)pg/ml比CC:(37.1±7.6)pg/ml,TT:(30.6±1.5)pg/ml]、T/C中的TT[TT:(43.3±5.3)pg/ml比TC:(32.3±1.3)pg/ml,CC:(30.7±3.7)pg/ml]等三种基因型的高血压患者的sCD40水平均较其他相应基因型显著升高(P<0.05～0.01);有A/G中的GG[GG:(9.5±0.3)m/s比AA:(9.0±0.1)m/s,GA:(8.8±0.1)m/s]、C/T中的CC[CC:(9.6±0.3)m/s比CT:(8.9±0.1)m/s,TT:(9.0±0.5)m/s]、T/C中的CC[CC:(9.3±0.2)m/s比TT:(9.0±0.1)m/s,TC:(8.9±0.1)m/s]等三种基因型的高血压患者的PWV均较其他相应基因型明显增快(P均<0.05)。结论:CD40的三种基因多态性与血清CD40浓度、脉搏波速率有关。

Objective：To study CD40 gene polymorphism of patients with essential hypertension（EH） related with serum level of CD40 and pulse wave velocity（PWV）.Methods：A total of 432 EH patients were enrolled in the study.Enzyme linked immunosorbent assay method was used to measure serum level of soluble CD40（sCD40）.Taqman polymerase chain reaction（PCR） method was used to measure CD40 gene polymorphism（A/G,C/T,T/C）.The relations of gene polymorphism of EH patients with serum level of CD40 and PWV were analyzed.Results：Compared with other corresponding genotypes,there were significant increase in sCD40 level in EH patients with CD40 GA genotype in A/G,CT genotype in C/T and TT genotype in T/C,P〈0.05~〈0.01.Compared with other corresponding genotypes,there were significant increase in PWV in EH patients with CD40 GG genotype in A/G,CC genotype in C/T and CC genotype in T/C,P〈0.05 all.Conclusion：Gene polymorphisms of CD40 are related with serum concentration of CD40 and pulse wave velocity.