川芎嗪对草犬肾脏低温保存的实验研究

Experimental research on the effect of Ligustrazine on canine kidney Cryopreservation

目的研究川芎嗪对犬肾脏低温保存的影响。方法将草犬分为四组,分别用高渗枸橼酸盐嘌呤溶液(HC-A液)及含川芎嗪(4 mg/L)的HC-A液对供肾进行灌注和保存24、48 h,观察肾脏形态学的改变,并进行自体肾脏移植,观察移植后肾功能恢复情况。结果含川芎嗪的HC-A液组低温保存48 h后肾脏形态学改变、移植肾功能指标明显优于HC-A液组(P<0.05),保存24 h两组差异无统计学意义(P>0.05)。结论川芎嗪对低温保存较长时间的肾脏质量有明显改善作用。

Objective To study the application of Ligustrazine in the kidney preservation solution.Methods We used an isolated perfused canine kidney model.Twenty canines were randomly divided into 4 groups:in the group I and II,the kidneys were flushed with HC-A solutions,and in the group III and IV,the kidneys were flushed with HC-A solutions which Ligustrazine was added(4 mg/L).After 24 h and 48 h preservation at 4 C,kidneys underwent histological analysis.In transplantation test,the renal function indices were assessed.Results Kidney histological changes and the renal function indices in the group IV were significantly superior to that of the group II(P<0.05).Conclusion Ligustrazine could improve the quality of donor kidney that preserved for a longer time.