广西甘蔗花叶病SCMV调查初报

Investigation of Sugarcane Mosaic Virus Infecting Sugarcane in Guangxi

为探明甘蔗花叶病SCMV在广西蔗区的发生情况,给甘蔗健康种苗生产提供科学依据,从广西各主要蔗区对不同品种采集表现花叶症状的甘蔗样品,采用1对病毒CP基因引物(SCMVF4:5′-GTTTTYCACCAAGCTGGAACAGTC-3′;Y=CorT,SCMVR3:5′-AGCTGTGTGTCTCTCTGTATTCTC-3′),进行一步法RT-PCR检测。结果表明:69.4%的样品为阳性。选取8份代表性样品,对经SCMVF4/SCMVR3扩增获得的病毒CP基因片段进行直接测序,所得序列经Blast比对确认均为SCMV CP序列。在所调查的品种中,主栽品种ROC22最易受SCMV侵染,其它台糖系列如台糖16、台糖28、台糖95-889、台优,柳城03-182、柳城03-1137及广西甘蔗研究所许多品系均检测到SCMV。目前甘蔗花叶病SCMV已在广西主要蔗区普遍发生。

In order to understand the occurrence of sugarcane mosaic virus（SCMV）,and to provide a basic reference for producing disease-free sugarcane seedlings in Guangxi.Sugarcane leaf samples with mosaic symptom were collected from cane growing fields in Guangxi.The virus was detected by one-step RT-PCR with SCMV specific primers（SCMVF4：5′-GTTTTYCACCAAGCTGGAACAGTC-3′;Y = C or T,SCMVR3：5′-AGCTGTGTGTCTCTCTGTATTCTC-3′）.The test results showed that 69.4% samples were found as a positive.BLAST analysis from eight amplified cDNA products revealed that they were all homologous SCMV CP gene.Investigation results indicated that ROC 22,the major variety in Guangxi was seriously infected by SCMV,and the other varieties such as ROC16,ROC28,ROC95-889,Taiyou,Liucheng03-182,Liucheng03-1137 and some strains from Guangxi sugarcane research institute were detected as a positive by RT-PCR.