摘要
目的 观察还原型谷胱甘肽（glutathione,GSH）在肺内源性急性肺损伤（pulmonary acute lung injury,ALIp）中的保护作用.方法 建立脂多糖（LPS）诱导的大鼠ALIp模型,24只SD大鼠随机分为对照组（NS组）、LPS组、GSH1组和GSH2组,血气分析法计算氧合指数（PaO2/FiO2）,测定各组肺湿/干质量比值（W/D）,双缩脲法测定支气管肺泡灌洗液（BALF）中蛋白含量,ELISA法测定IL-1β、IL-6的含量,硫代巴比妥酸法测定丙二醛（MDA）;采用黄嘌呤氧化酶法测定超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）.结果 GSH1组和GSH2组大鼠肺湿/干质量比值,IL-1β、IL-6含量,BALF中蛋白含量,MDA含量均小于LPS组（P〈0.05）.GSH1和GSH2组中SOD、GSH-Px含量高于LPS组（P〈0.05）.GSH1组和GSH2组间差异无统计学意义（P〉0.05）.结论 GSH能通过阻断细胞因子和炎性介质的释放,对抗氧自由基作用来抑制ALIp大鼠的肺部炎症反应;但增大GSH剂量并不能增强其对ALIp的保护作用.
Objective To observe the protective role of reduced glutathione（ GSH ） on pulmonary acute lung injury （ALIp）. Methods To establish the model of ALIp induced by lipopolysaecharide （LPS） , 24 SD rats were randomly di- vided into four groups ： NS group, LPS group, GSH1 group and GSH2 group. To determine the PaOJFiO2 index by blood gas analysis. Broehoalveolar lavage fluid （BALF） in each group was collected and wet to dry weight ratio （W/D） was determined. Interleukin- 1 β （IL- 113） and interleukin- 6 （IL- 6 ） were determined by ELISA method, the protein in brochoalveolar lavage fluid （BALF） was determined by Biuret method. The determination of malondialdehyde （MDA） by sulfur acid and benzodiazepines method, and superoxide dismutase （SOD） by xanthine oxidase method, and so as glutathione peroxidase （ GSH - Px）. Results The level of W/D ratio, IL - 113, IL - 6, the content protein in BALF and the MDA in lung tissue in GSH group were all significantly lower than that in LPS group （ P 〈 0. 05 ）. The content of SOD and GSH - Px in lung tissue in GSH group were much higher than that in LPS group （ P 〈 0. 05 ）. But there was no difference between group GSH1 and group GSH2 （P 〉 0. 05）. Conclusion GSH can block the release of cells factor and inflammatory mediators, against oxygen free radical action so as to curb the inflammation of ALIp in rats. But increasing the GSH dose cannot increase the protection of ALIp.
出处
《徐州医学院学报》
CAS
2012年第1期6-9,共4页
Acta Academiae Medicinae Xuzhou
基金
基金项目：江苏省“六大人才高峰”第六批资助项目（2009059）
