泛影葡胺加重肾毒性及其对肾组织骨形成蛋白7表达的影响

Expression and significance of BMP -7 in renal tissues of rat with nephrotoxicity aggravated by lodipamide meglumine

目的 探讨高渗造影剂对甘油所致肾损害的大鼠肾毒性作用及可能机制.方法 32只SD大鼠随机分为正常对照组（N组）、甘油对照组（G组）、泛影葡胺对照组（LM组）、甘油＋泛影葡胺组（G＋LM组）,每组8只.G组和G＋LM组给予50%的甘油,后腿深部肌肉注射;N组和LM组以同样的方法给予等体积的生理盐水.3天后,LM组和G＋LM组再给76%泛影葡胺（10 ml/kg）尾静脉注射;N组和G组按同样方法给予等量的生理盐水.各组于5天后检测血清肌酐（SCr）水平,苏木精-伊红染色观察肾组织病理损害,免疫组织化学法检测肾组织骨形成蛋白7（BMP-7）的表达.结果 BMP-7在N组肾组织中有高度表达,G组表达减少,LM组有中度表达,而G＋LM组表达明显减少.结论 泛影葡胺能加重SD大鼠原有的急性肾毒性,骨形成蛋白7表达的下调是肾损伤的加重的重要因素之一.

Objective To explore the possible effect and mechanism of nephrotoxicity of high - osmolar which applys in renal injury that glycerol induced. Methods 32 SD rats were randomly divided into normal control group （group N） , glycerin control group （ group G） and lodipamide meglumine control group （ group LM ） , glycerin ＋ lodipamide meglumine groups （G ＋ LM） （n = 8/group）. G and G ＋ LM two groups, given 50% of glycerin levels by 10 ml/kg weight in hind - leg muscles injection, equal volume of normal saline （ 10 ml/kg） was injected through hind - leg muscles in rats from N and LM groups. Three days later, LM and G ＋ LM groups was injected 76% of lodipamide meglumine （ 10 ml/kg） through tail vein and equal volume of normal saline （ 10 ml/kg） did like the former from N and G groups. Serum ereatinine （SCr） level was detected; The renal injury was assessed by hematoxylin and eosin （HE）staining; The expression of BMP -7 in renal tissues was detected by immunohistoehemistry assay. Results Expression of BMP -7 in the N group was in high level and that in the G group was less. The expression of BMP - 7 in the LM group was a little shorter than that in the N group, but the expression in G ＋ LM group was significantly lower than other groups. Conclusion The nephrotoxicity of original acute renal injury induced by lodipamide meglumine is increased, and the expression of BMP - 7 is lower and that is one of the important reasons about increasing of renal injury.