白鹅脂联素基因内含子多态性检测及群体遗传分析

Identification and genetic analysis of polymorphism in intron of adiponetin gene in White goose

以扬州鹅、四川白鹅、皖西白鹅、豁眼鹅和浙东白鹅5个鹅品种为试验材料,每个品种各60只,公母各半,根据鹅脂联素基因序列设计了1对引物扩增部分内含子,用PCR-SSCP方法进行单核苷酸多态性分析,并对不同品种进行群体遗传学分析。结果表明,在鹅脂联素基因内含子上发现2个单碱基突变位点,分别为第16、26位点的A-G、T-C。鹅群中表现出3种基因型(AA、AB、BB);除豁眼鹅与皖西白鹅、浙东白鹅外,3种基因型在鹅品种两两之间分布无显著差异(P>0.05)。群体遗传分析表明,豁眼鹅杂合度和多态信息含量最高,皖西白鹅最低,所有品种均表现为中度多态性。本研究表明鹅脂联素基因在不同品种中具有丰富的单核苷酸多态性,可以进一步作为候选基因来分析其与脂肪性状的关联性。

Five breeds of geese（Yangzhou goose,Sichuan White goose,Wanxi White goose,Huoyan goose and Zhedong White goose）with 30 male and 30 female each were used in this experiment.One pair of primer was designed for amplification of intron fragment based on adiponectin gene sequence of goose,and then single nu-cleotide polymorphism（SNP） and genetic polymorphism in different breeds were analyzed by PCR-SSCP method.As a result,two single nucleotide polymorphism mutations（A16G and T26C） were found in adiponectin gene and three genotypes（AA,AB,and BB） were detected in the five breeds.Except for that between Huoyan goose and Wanxi White goose,and Huoyan goose and Zhedong White goose,the distribution of three genotypes was not significantly different between every two breeds.The heterozygosity and PIC（polymorphism information content） were highest in Huoyan goose,but lowest in Wanxi White goose.Every breed′ s PIC was in the middle level.The results showed that the adiponectin gene has high polymorphisms in goose breeds,and could be used as a candi-date gene for analyzing the correlation between its polymorphism and adipo traits in goose.