摘要
将已克隆的一个被冷显著上调的基因CsCOR1整合到穿梭载体pBI121的CaMV35S启动子的下游,然后采用农杆菌介导法转化进烟草细胞,从而制得了转CsCOR1基因烟草植株,并筛选到组成型表达CsCOR1基因的烟草转化子,为进一步研究该基因的功能奠定了基础.
CsCOR1,a gene upregulated remarkably by cold,was inserted CaMV35S promoter behind of pBI121 vector,producing a pBI121-CsCOR1.Then,the vector was introduced Agrobacterium tumefaciens strain EHA105 using freeze-thaw transformation.Transgenic-tobaccos were generated by Agrobacterium-mediated transformation using the leaf disc system,and two plants overexpressing CsCOR1 gene were screened.The harvests lay the foundation of CsCOR1 function research.
出处
《信阳师范学院学报(自然科学版)》
CAS
北大核心
2012年第1期64-66,共3页
Journal of Xinyang Normal University(Natural Science Edition)
基金
河南省科技攻关项目(082102150009)
信阳师范学院博士启动基金项目
关键词
表达载体构建
遗传转化
烟草转化子
超表达
constructing expression vector; genetic transformation; tobacco transformants; overexpression