分子信标荧光定量PCR在肺结核早期诊断及疗效评价中的初步应用

Preliminary study on early diagnosis and efficacy evaluation of pulmonary tuberculosis based on molecular beacon real-time PCR

目的探讨分子信标荧光定量PCR在肺结核早期诊断及疗效评价中的应用价值。方法采集2010年3—7月间在彭州市CDC结核病防治所门诊就诊患者的痰标本382份,其中初诊疑似肺结核患者的痰标本305份,肺结核患者随访痰标本77份,用分子信标荧光定量PCR法和抗酸染色法检测Mtb。结果382份痰标本抗酸染色法、分子信标荧光定量PCR法检测总阳性率分别为12.6%(48/382)、42.4%(162/382),分子信标荧光定量PCR法阳性率显著高于抗酸染色法,差异有统计学意义(χ2=74.5,P<0.001)。结论分子信标荧光定量PCR较抗酸染色法具有更高的灵敏度,不仅提高了肺结核早期诊断的检出率,还能间接反映药物疗效,在肺结核防治工作中具有重要意义。

Objective To investigate early diagnosis and efficacy evaluation of pulmonary tuberculosis using molecular beacon real-time polymerase chain reaction（PCR）.Methods Molecular beacon real-time PCR and acid-fast staining were used to detect Mycobacterium tuberculosis in 305 sputum specimens collected from pulmonary tuberculosis suspects at initial testing and 77 sputum specimens collected from pulmonary tuberculosis patients.Results Using acid-fast staining and molecular beacon real-time PCR,the positive rates were 12.6%（48/382）,42.4%（162/382）,respectively.The positive rates of detection of molecular beacon real-time PCR was significantly higher than that of acid-fast staining（χ2=74.5,P〈0.001）.Conclusion Compared with acid-fast staining,molecular beacon real-time PCR shows higher sensitivity in detection of pulmonary tuberculosis.It enhances the detection rate and indirectly reflects efficacy of medications,thus showing promising in control and prevention of tuberculosis.