槲皮素对CBRH-7919细胞增殖及线粒体膜电位的影响

Effect of Quercetin on the Proliferation and Mitochondrial Transmembrane Potential of CBRH-7919 Cells

[目的]探讨槲皮素对肝癌细胞株CBRH-7919增殖及线粒体膜电位的影响。[方法]体外培养肝癌CBRH-7919细胞,以不同浓度槲皮素处理细胞后,采用酸性磷酸酶(APA)法检测OD405;倒置显微镜观测CBRH-7919细胞形态变化;Rhodamine 123染色后流式细胞仪检测线粒体膜电位(△ψm)变化。[结果]槲皮素对肝癌CBRH-7919细胞有明显的增殖抑制作用,并与剂量和作用时间成正比;光学显微镜下可见槲皮素作用后细胞密度明显降低;经10μg/ml槲皮素作用122、4、48 h后,流式细胞术检测到Rhodamine 123染色弱荧光细胞百分比逐步增加,线粒体膜电位下降。[结论]槲皮素体外能抑制肝癌细胞株CBRH-7919增殖,引起线粒体膜电位下降。

[Objective] To investigate the effect of quercetin on the proliferation and mitochondrial transmembrane potential of CBRH-7919 cells.[Method] The CBRH-7919 cells of hepatocarcinoma were cultured in vitro.After treated with different concentrations of quercetin,the OD405 of CBRH-7919 cells was detected by using the acid phosphatase assy（APA）;morphologic changes of the cells were observed under inverted microscope;the mitochondrial transmembrane potential（△ψm） intensity changes of CBRH-7919 cells were analyzed by flow cytometry after stained with Rhodamine 123.[Result] Quercetin inhibited the proliferation of CBRH-7919 cells significantly,and the growth inhibitory effect presented time and dose-dependent relationship.Typical decrease of cell density was observed by optical microscopy on the quercetin treated cells.With the effect of 10 μg/ml quercetin on CBRH-7919 cells for 12,24 and 48 h,the percentage of Rhodamine 123 stained hypofluorescence cells increased,while the mitochondrial transmembrane potential（△ψm）intensity of CBRH-7919 cells decreased.[Conclusion] Quercetin could inhibit the proliferation of CBRH-7919 cells in vitro,causing decrease in mitochondrial transmembrane potential.