活性污泥胞外聚合物提取条件的优化

Optimization of Extraction Condition of Extracellular Polymeric Substances from Activated Sludge

[目的]探索活性污泥胞外聚合物(EPS)提取的最佳条件。[方法]比较研究了5种方法(H2SO4法、甲醛-NaOH法、搅拌法、热提法和NaOH法)对活性污泥EPS的提取效果,同时确定了最适提取方法的最优提取条件。[结果]5种提取方法中NaOH法因其提取的DNA含量较少,提取时间较短而比较适宜。以NaOH法提取EPS,在pH为11,提取时间为10 min,搅拌速度在80～120 r/min下提取效率最高。[结论]确定EPS的最适提取条件可为其研究提供理论基础。

[Objective] To explore the optimal extraction conditions of extracellular polymeric substances（EPS） from activated sludge.[Method] The efficiency of five methods（H2SO4,formaldehyde-NaOH,mixing,heating and NaOH） on the extraction of EPS was investigated comparatively.The optimal extraction conditions of the most suitable method were determined.[Result] NaOH method is the most effective in extracting EPS as DNA was less and the extraction time was shorter.The optimal extraction condition was pH of 11,extraction time of 10 minutes and agitation speed of 80-120 r/min.[Conclusion] The determined optimal extraction condition provided theoretical basis for EPS study.