HBD-2和IFN-γ在寻常型银屑病皮损中的表达及其意义

Expression and the significance of HBD-2 and IFN-γin the lesions of psoriasis vulgaris

目的研究HBD-2、IFN-γ在寻常型银屑病皮损中的表达并探讨其意义。方法采用实时荧光定量RT-PCR法检测45例寻常型银屑病患者皮损组织、非皮损组织和15例非银屑病患者正常皮肤组织中的HBD-2和IFN-γ的mRNA表达水平,按PASI评分标准将皮损组分为轻、中、重组,检测HBD-2和IFN-γmRNA在银屑病皮损不同组中的表达水平。结果银屑病患者皮损区HBD-2、IFN-γ的表达明显上调,非皮损区HBD-2、IFN-γ的表达也高于正常皮肤组织,差异均有统计学意义(P<0.05);HBD-2、IFN-γ在轻中重不同皮损中的表达表达依次增高,差异有统计学意义。IFN-γ与HBD-2存在正相关。结论 IFN-γ和HBD-2可能共同参与了寻常型银屑病的皮损形成。

Objective to explore the expressions HBD-2 and IFN-γ in the lesional skin of Psoriasis Vulgaris and discuss the significance.Methods Skin lesions of psoriasis vulgaris organization was divided into mild,moderate and severe groups based on PASI score rule,then Real-time fluorescent quantitative reverse transcriptase polymerase chain reaction technique was used to detect the mRNA expressions level of IFN-γ,HBD-2 among 45 psoriasis lesions、 non-lesional psoriasis skin tissue and 15 normal skin tissue,also in skin lesions of psoriasis vulgaris organization diffent groups.Results The mRNA expression of HBD-2 and IFN-γwas obviously up-regulated in the lesional skin,non-lesional psoriasis skin was higher than normal skin tissues controls,and was different in diffent lesions of psoriasis groups.the expression of IFN-γhad positive correlation with that of HBD-2.Conclusion IFN-γ and HBD 2 together may contribute to the pathogenesis of Psoriasis Vulgaris