酶联免疫法测定猪肉、猪肝和猪尿中盐酸克伦特罗

Enzyme Linked Immunoassay Determination of Clenbuterol Hydrochloride in Pork,Pork Liver and Pig Urine

对4种国产及进口的酶联免疫法测试盒测定猪肉、猪肝及猪尿中克伦特罗残留量的性能及效果作了对比试验。试验时以上述3种样品为基体,加入1,2,3,5,10μg.kg-1(或μg.L-1)等5个浓度水平的克伦特罗标准溶液,然后用上述测试盒进行检测。从所得的结果可知,国内外的测试盒所得测定值间存在较大差异;猪尿样品的检测结果表明这些测试盒所采用的方法比较适合,但由猪肉及猪肝样品的检测结果可知,其中一些测试盒所采用的方法尚有改进的必要。

Four native-made and imported testing cases for enzyme-linked immunoassay（ELIA） were studied comparatively for their efficiency in determination of residual amount of clenbuterol in pork,pork liver and pig urine.Standard solutions of clenbuterol at concentration levels of 1,2,3,5 and 10 μg·kg-1（or μg·L-1） were added to 3 different samples mentioned above and tested separated.As shown by the results of testing,① quite obvious deviations were found between the native-made and imported testing cases,and ② the testing cases were relatively suitable for use in the ELIA of pig urine samples,while for the ELIA of the tissue samples,modifications for the methods used in some of the testing cases were found to be necessary.