气相色谱法测定果蔬中防腐剂仲丁胺残留量

GC Determination of Residual Amount of the Preservative Butylamine in Fruits and Vegetables

提出了气相色谱法测定果蔬中防腐剂仲丁胺残留量的方法。果蔬样品经匀浆处理后置于顶空瓶中,在碱性条件下于55℃恒温平衡40min。吸取100μL液上气体注入气相色谱仪,用氮磷检测器测定,外标法定量。仲丁胺的质量浓度在0.000 5～0.040 0mg.L-1范围内与其峰面积呈线性关系,检出限(3S/N)为0.5μg.L-1。在两个浓度水平上对方法进行回收试验,测得回收率在87.4%～102.8%之间,测定值的相对标准偏差(n=10)在2.6%～9.6%之间。

A method of GC for determination of residual amount of the preservative butylamine in fruits and vegetables was proposed.After pounding and homogenization,the sample of fruit or vegetable was weighed into a head-space bottle,and equilibrated for 40 min in an alkaline solution at 55 ℃.A portion of 100 μL of the gas over the liquid solution was taken and injected into the instrument for GC analysis,with NPD and external standard calibration.Linear relationship between values of peak area and mass concentration of butylamine was obtained in the range of 0.000 5-0.040 0 mg·L-1,with detection limit（3S/N） of 0.5 μg·L-1.Recovery was tested at 2 concentration levels,the results found were in the range of 87.4%-102.8%,with values of RSD（n=10） ranged form 2.6%-9.6%.