摘要
A curcumin derivative ligand,1,7-bis(3-methoxyl-4-acetoxyl)phenyl-1,6-heptadiene-3,5-diketone (diacetylcurcumin,abbreviated as HL),and its Cu and Ni complexes have been synthesized and fully characterized by elemental analyses,IR,1 HNMR and molar conductivity.The resulting complexes exhibit two-photon excited fluorescence (TPEF) in DMF,and have been proven to be potentially useful for two-photon microscopy imaging in living cells.In addition,cytotoxicity tests showed that the low-micromolar concentrations of ML 2 did not cause significant reduction in cell viability over a period of at least 24 h and should be safe for further biological studies.
A curcumin derivative ligand, 1,7-bis（3-methoxyl-4-acetoxyl）phenyl-1,6-heptadiene-3,5-diketone （diacetylcurcumin, abbrevi- ated as HL）, and its Cu and Ni complexes have been synthesized and fully characterized by elemental analyses, IR, 1HNMR and molar conductivity. The resulting complexes exhibit two-photon excited fluorescence （TPEF） in DMF, and have been proven to be potentially useful for two-photon microscopy imaging in living cells. In addition, cytotoxicity tests showed that the low-micromolar concentrations of ML2 did not cause significant reduction in cell viability over a period of at least 24 h and should be safe for further biological studies.
基金
supported by the National Natural Science Foundation of China (21071001)
Department of Education Committee of Anhui Province (KJ2010A222)
the Natural Science Foundation of Anhui Unversity of Traditional Chinese Medicine (2011zr005A)
