摘要
目的:海洋细菌产几丁质酶培养条件优化。方法:在单因素试验的基础上,利用响应面法分析各因素之间的交互作用对几丁质酶产率的影响。结果:通过响应面试验得到最佳培养条件:温度为26.4℃,NaCl的百分浓度为3.39%,培养基初始pH值为6.3。结论:在最适产酶发酵条件下,菌株产几丁质酶的活性比优化前提高了5.4倍。
Objective: To optimize the chitinase production conditions of a marine baterium. Method: On the basis of single-factor test, the effects of the interactions of each 2 factors on chitinase production were analyzed by response surface methodology. Results: The optimum culture conditions were: temperature 26℃, NaC1 3.39% and initial pH value of medium 6.3. Conclusion: After optimization, the chitinase production increased about 5.4 times.
出处
《中国酿造》
CAS
2012年第2期103-106,共4页
China Brewing
关键词
响应面法分析
几丁质酶
培养条件
response surface methodology
chitinase
cultivation condition