响应面法优化海洋细菌产几丁质酶培养条件

Optimization of chitinase production conditons of a marine bacterium by response surface methodology

目的:海洋细菌产几丁质酶培养条件优化。方法:在单因素试验的基础上,利用响应面法分析各因素之间的交互作用对几丁质酶产率的影响。结果:通过响应面试验得到最佳培养条件:温度为26.4℃,NaCl的百分浓度为3.39%,培养基初始pH值为6.3。结论:在最适产酶发酵条件下,菌株产几丁质酶的活性比优化前提高了5.4倍。

Objective： To optimize the chitinase production conditions of a marine baterium. Method： On the basis of single-factor test, the effects of the interactions of each 2 factors on chitinase production were analyzed by response surface methodology. Results： The optimum culture conditions were： temperature 26℃, NaC1 3.39% and initial pH value of medium 6.3. Conclusion： After optimization, the chitinase production increased about 5.4 times.