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米曲霉种内原生质体融合选育优良菌株 被引量:8

Breeding Aspergillus oryzae strain by intraspecific protoplast fusion
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摘要 米曲霉HL和L5是来自酱油酿造企业的生产菌株。HL的生长速度快,而L5的中性蛋白酶活力高。以HL和L5为亲本,进行原生质体融合,筛选得到一株融合株R6。融合株比亲本株生长速度更快,孢子成熟时间快3h,中性蛋白酶活力分别比2株亲本菌株提高25.6%和19.9%,酱醅氨基氮值也比亲本菌株提高8%和5.6%。 Two Aspergillus oryzae strains (HL and L5) were often used in soy sauce brewing. HL strain grows fast while L5 strain had a high neutral protease activity. By using protoplast fusion technique, a fusion strain of R6 was obtained which grows faster and had a short period of spore maturation (3h) when using HL and L5 as parental strains. The neutral protease activity of R6 was increased by 25.6% and 19.9% compared to those of ilL and L5 strain, respectively. Amino nitrogen value of sauce mash was increased by 8% and 5.6% compared to those of ilL and L5 strain, respectively.
出处 《中国酿造》 CAS 2012年第2期132-136,共5页 China Brewing
关键词 米曲霉 中性蛋白酶 氨基态氮 原生质体融合 Aspergillus oryzae neutral protease amino nitrogen protoplast fusion
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