侗家奇液酒中游离氨基酸含量测定分析

Determination and analysis of free amino acids content in Dongjiaqiye wine

采用日立L-8800型氨基酸自动分析仪分析研究侗家奇液酒中游离氨基酸的组成，色谱条件：标准分析柱4．6mm×60．Omm，反应柱温度：150℃，测定了不同地区保健酒中游离氨基酸的含量。结果表明，所测试样中各种氨基酸加标回收率均在85．2％-112．6％，最低检出限为3pmol。该仪器重现性好，实验数据可靠。测定的侗家奇液酒中游离氨基酸总含量为488．48mg／L，仅次于灵芝酒；谷氨酸含量为252．12mg／L，占氨基酸总量的51．59％，在6种保健酒中含量最高，是一种天然绿色、营养健康的保健佳品。

The contents of free amino acids in the Dongjiaqiye wine from different region were determined by the Hitachi L-8800 amino acid mobile analyzer. The chromatographic condition were as followed： standard column 4.6mm×60.0mm and temperature of reactive column 150℃. The results showed that the recoveries of all samples were in the range 85.2%-112.6% and the lowest detection limit was 3pmol. It was indicated that the instru- ment reproduction was good and the experimental data was reliable. The totals free amino acids in Dongjiaqiye wine was 488.48mg/L, which was only lower than that of Ganoderma wine. The glutamic acid content was 252.12mg/L which accounted for 51.59% of total amino acids, and its con- tent was highest in six kinds of health wine. It is a natural and nutritional food.