脂质体携载前列腺素E1对扩张皮瓣缺血一再灌注损伤的影响

Influence of liposome PGE1 on ischemia-reperfusion injury of expanded flap

目的观察脂质体携载前列腺素E1（1iposome prostaglandin E1，LipoPGE1）对扩张皮瓣缺血再灌注损伤的影响。方法采用中国小型猪背部扩张随意皮瓣缺血一再灌注损伤模型，实验分为两组：实验组（LipoPGEl干预组）及对照组。在其背部形成8cm×2cm扩张皮瓣，通过不同时间远端扩张皮瓣组织匀浆中髓过氧化酶（MPO）及丙二醛（MDA）含量测定，以及皮瓣微血管密度（MVD）、皮瓣存活率和皮温测定，观察LipoPGEl对皮瓣成活的影响。结果实验组皮瓣存活率明显高于对照组（P％0．05），术后两组MPO及MDA含量差异有统计学意义（P〈0．05），实验组明显低于对照组。两组MVD含量差异无统计学意义（P〉0．05）。实验组用药后皮肤温度明显升高。结论应用LipoPGEl可以减轻缺血一再灌注损伤，提高扩张皮瓣存活率。

Objective To investigate the influence of the liposome PGEI on the ischemia-reper- fusion injury of the expanded flap. Methods The minipigs were set up as an animal model. They were divided into 2 groups. The expanded flaps （8 cm × 2 cm ） was designed and elevated on the back of the minipigs. An image analysis technology was used to evaluate the survival rate of the expanded flap, while a biochemistry method was used to detect the concentrations of malondialdehyde （MDA）, myeloperoxidase （MPO） and microvessel density （MVD） in the tissue. Skin tempreture was measured after the drug administration. Results The survival area of the expanded flap in the Lipo PGE1 group was significantly enlarged （P〈0.05）, compared with the control group. The MPO and MDA concen- tration in the tissue was statistically different between two groups （P〈 0. 05）, but there was no difference of the MVD concentration between two groups. The skin temperature was higher in the Li- po PGE1 group. Conclusions The Lipo PGE1 could promote survival rate in the expanded flap and protect it from ischemic injury.