小麦钙联蛋白基因TaCNX600.的克隆与表达分析

Molecular cloning of wheat calnexin gene TaCNX60.0 and analyzing of its expression

钙联蛋白(Calnexin)是一类结构和功能非常保守的分子伴侣,在调控生物代谢和Ca2+信号传导等方面发挥重要作用。本研究通过筛选Yr5近等基因系cDNA文库,分离获得1个钙联蛋白基因,将其命名为TaCNX600.。序列分析发现,该cDNA片段包含1个1 921bp的开放阅读框,推测其编码包含535个氨基酸残基的蛋白质。利用半定量RT-PCR分析该基因的表达谱,发现小麦受到植物激素ABA、低温和干旱胁迫处理后,TaCNX600.的表达受到抑制,而条锈菌小种CYR32和白粉菌混合菌系侵染能诱导TaCNX600.表达量增加,说明小麦钙联蛋白基因TaCNX600.可能在植物防卫反应和抵抗逆境胁迫过程中发挥作用。

Calnexin is one of the molecular chaperone molecules with highly conserved structures and biological functions.It plays crucial roles in the regulation processes of biological metabolism and Ca2＋ signal transduction.In this study,we screened a cDNA library of a near-isogenic line of wheat stripe rust resistance gene Yr5 and cloned a calnexin gene named TaCNX60.0.Sequence analysis showed that the cDNA fragment contained a 1 921-bp open reading frame,and was predicted to encode a protein with 535 amino acids.Semi-quantitative RT-PCR was performed to investigate the gene expression profiles when wheat plants were treated with different stress conditions and after inoculated with wheat pathogens.It was found that TaCNX60.0 expression was suppressed by exogenous phytohormone ABA,cold and drought treatments.However,it was induced after infection by a strain of Puccinian striiformis CYR32 and mixed races of Erysiphe graminis f.sp.triticis.These results indicated that TaCNX60.0 may involve in wheat defense and stress responses.