碳氮源对灵芝液体发酵胞外糖肽产量的影响

Effect of carbon and nitrogen source on exopolysaccharide peptides production of Ganoderma lucidum in submerged culture

为寻找适合灵芝胞外糖肽(EPSP)液体深层发酵工艺商业化生产的经济高效的培养基原料,在摇瓶水平上比较研究了不同类型的碳源和氮源(各6种)对灵芝胞外糖肽(EPSP)产量的影响。试验表明:玉米粉是最适合灵芝EPSP生产((2.664±0.801)g/L)和菌丝生长((4.559±0.150)g/L)的碳源;尿素是最适合灵芝EPSP生产((0.636±0.040)g/L)的氮源,豆粕粉则是最适合灵芝菌丝生长的氮源((2.222±0.256)g/L);玉米粉与豆粕粉组合是最适合灵芝EPSP生产((4.366±0.434)g/L)的碳氮源组合。本研究结果可为灵芝EPSP液体深层发酵工艺的工业化生产提供科学指导。

The purpose of this study is to discover high efficient and economical raw materials for commercial production of G.lucidum exopolysaccharide peptides（EPSP） in submerged culture.Twelve different souces（six carbon and six nitrogen sources） were used individually in 250 mL shake flask.The optimal carbon source was cornmeal for both EPSP production（（2.664±0.801） g/L） and cell growth（（4.559±0.150） g/L）,the best nitrogen source was urea for EPSP production（（0.636±0.040） g/L）,and the most favorable nitrogen source was soybean powder for cell growth（（2.222±0.256） g/L）.Subsequently,the most advantageous combination in EPSP yield was cornmeal＋soybean powder,which resulted in up to（4.366±0.434） g/L.The result of this study would be beneficial to industrial production of EPSP from G.lucidum.