不同强度永磁磁场对成纤维细胞的生物学活性影响研究

Influence of various magnetic field intensity on the viability of fibroblast cells

目的以磁源空间磁场定量计算为依据,研究正常培养条件下与缺氧培养条件下不同强度永磁磁场对人皮肤成纤维细胞的影响。方法选用人皮肤成纤维细胞,以5×104/mL密度每孔100μL种于96孔板。磁源N面中心实测磁感应强度分别为3.4、5.6、13.8、27.8、52.6、107.0、178.9、292.2 mT的8组磁源,前6组(钐钴合金)尺寸分别为Φ8.0 mm×2.0 mm,后两组(钕铁硼)尺寸分别为Φ8.0 mm×1.5 mm、Φ8.0 mm×2.5 mm,均轴向充磁,即为A、B、C、D、E、F、G、H组。将8组磁场分别作用于正常培养条件下与缺氧培养条件下的人皮肤成纤维细胞,并设对照组(加磁组与非加磁组),3 d后用四甲基偶氮唑盐(MTT)法分别检测细胞活力,做统计学分析。结果两种培养条件下8组不同强度的加磁组人皮肤成纤维细胞的MTT数值与非加磁组的对照组相比较均有升高,正常培养中磁场强度为B、C、D、E、F、G、H组与对照组相比较,差异有统计学意义(P<0.05),且随着磁场强度的增加活性值越高;缺氧培养中磁场强度为G、H组与对照组相比较,差异有统计学意义(P<0.05)。结论实验中选取的永磁磁场对于人皮肤成纤维细胞有增殖和降低损伤的作用。

Objective To study the effect of permanent magnetic fields on human skin fibroblast cells cultured under normal or hypoxic condition,and explore the dose-effect based on the calculated intensities of space magnetic fields.Methods A total of 100 μL of human skin fibroblast cells were plated into each well of a 96-well microplate at a density of 5 × 104/mL.N-magnetic fields with 8 different intensities were employed in the present study： 3.4 mT,5.6 mT,13.8 mT,27.8 mT,52.6 mT,107.0 mT,178.9 mT,and 292.2 mT.The size of group A,B,C,D,E,F（samarium-cobalt alloy） was Φ8.0 mm × 2.0 mm,and the size of group G and H（NdFeB） were Φ8.0 mm × 1.5 mm and Φ8.0 mm × 2.5 mm,all of them were axially magnetized.Human skin fibroblasts were treated with the magnetic fields under normal or hypoxic conditions,with the untreated cells as controls.After 3 days,the viability of the fibroblast cells were measured by methyl thiazolyl tetrazolium salt（MTT） method.Results The cell viability of 8 groups under 2 different culture conditions were higher than that of the control group.Under normal culture condition,the cell viability of B,C,D,E,F,G and H groups were significant higher as compared with the control group（P 0.05）,and the cell viability increased with the increase of magnetic field intensity.Under hypoxic culture condition,the cell viability of G and H groups were significant higher than that in the control group（P 0.05）.Conclusion It is demonstrated that the magnetic fields have a proliferation-promoting effect on human skin fibroblast cells.