响应面法优化火焰原子吸收仪检测Zn元素工作条件

Optimization of testing conditions of Zn element using flame atomic absorption though response surface methodology

采用”响应面法优化火焰原子吸收仪检测Zn元素工作条件、通过在单因素基础上，用Design-Expert8．0软件中Box—Behnken Design试验设计研究灯电流、光谱通带、乙炔流量、燃烧器高度4个变量对Zn元素吸光度值的影响程度，用响应面法得出4个考察因素最优工作参数和吸光度预测值：灯电流1．0mA、光谱通带0．26 nm、乙炔流量1302．01mL／min、燃烧器高度6．29ram．吸光度预测值0．356Abs。通过实验证实：吸光度真实值与预测值接近，时样品检测，样品平均回收率在93％～104％之间，RSD在0．18％～0．26％范围内.

To optimize testing conditions of Zn element using flame atomic absorption spectrophotometers. Effects of lamp current, width of spectal bandpass, acetylene flow and burner height was investigated respectively in single-factor tests on absorbance of Zn element and Optimized by the maximization of the yield through response surface method ology with four factors and three levels based on the principle of Box-Behnken design. The results showed that the optimal testing conditions were as follows： elementslamp current 1.0mA, width of spectal bandpass 0.26nm, acetylene flow 1302.01mL/min, burner height 6.291nm and the predicted value of absorbance was 0.356Abs. Under the above conditions, the continued tests indicated that realistic and predicted value of absorbance was close, the relative standard deviations（RSD）were in the range of 0.18%-0.26% and the recovery were in the range of93%-104% .