摘要
目的:探讨大鼠血管平滑肌细胞(VSMCs)结缔组织生长因子(CTGF)表达水平在高血压血管重构中的变化及其意义。方法:以4周龄及16周龄的自发性高血压大鼠(SHR)为模型,以相同周龄的Wistar-Kyoto(WKY)大鼠为正常对照,采用tail cuff法测量SHR及WKY大鼠尾动脉收缩压。开胸后分离胸主动脉,分别测量胸主动脉中层厚度(M)和管腔内径(L),并计算二者的比值(M/L)。应用免疫荧光技术结合激光共聚焦显微镜观察,对CTGF的表达进行定位及定量检测。采用Western blot分析和实时定量RT-PCR,检测不同周龄的SHR主动脉组织内CT-GF、III型胶原(Col III)蛋白及其mRNA的表达。结果:4周龄SHR主动脉M、L、M/L以及ColⅢ蛋白与mRNA表达水平较同龄的WKY相比,均无显著性差异;但CTGF蛋白及mRNA表达水平均明显增高(P<0.05)。16周龄的SHR与同龄的WKY大鼠相比,胸主动脉的M无明显变化;而L显著增高,M/L显著降低(P<0.01);CTGF和ColⅢ的表达亦显著升高(P<0.01)。结论:实验结果提示,异常的血流动力学因素可调节VSMCs中CTGF的表达,从而引起细胞外基质释放增加,导致血管重构的发生。
AIM:To explore the varied expression level of connective tissue growth factor(CTGF) in rat vascular smooth muscle cells(VSMCs) and its significance.METHODS:Using age-matched Wistar-Kyoto(WKY) rats as control,the lumen diameter(L),media thickness(M),and media/lumen ratio(M/L) from 4-week-old and 16-week-old SHR were detected,respectively.Protein expression in vascular cells of WKY rats and SHR was then detected by immunohistochemical techniques and Western blot,and mRNA level was analyzed by real-time reverse transcription-polymerase chain reaction with SYBR(RT-qPCR).RESULTS:The ratio of L,M and M/L in 4-week-old SHR were similar to that in 4-week-old WKY rats(P0.05),and the protein and mRNA level of Col III in vascular cells of 4-week-old SHR were also similar to that of age-matched rats WKY rats.CTGF expression was upregulated significantly(P0.05).In 16-week-old SHR,the ratio of M/L were lower compared with age-matched WKY rats(P0.01).Protein and mRNA levels of Col III and CTGF in 16-week-old SHR were significantly higher than that of 16-week-old WKY(P0.01).CONCLUSION:Abnormal hemodynamic changes may regulate CTGF expression in VSMCs.Increased expression of CTGF may contribute to vascular remodeling in hypertension.
出处
《心脏杂志》
CAS
2012年第1期1-6,共6页
Chinese Heart Journal
基金
国家自然科学基金项目资助(30900617)
教育部博士点基金新教师基金项目资助(2008.6981036)
陕西省科技攻关项目资助[2007K13-03(13)]
国家重点研究发展基础计划(973)项目资助(2006CB503802)
关键词
结缔组织生长因子
高血压
血管重构
大鼠
connective tissue growth factor
hypertension
vascular remodeling
rat