HPLC法测定胎盘灌流液中安替比林浓度

Determination of the Concentration of Antipyrine in Placental Perfusion by HPLC

目的:建立测定胎盘灌流液中安替比林浓度的方法。方法:样品采用液-液萃取预处理后,以高效液相色谱法测定。色谱柱为WatersSymmetryC18,流动相为甲醇∶0.05mol·L-1磷酸氢二钠缓冲液(pH=6.5)=70∶30,内标为非那西丁,流速为1mL·min-1,紫外检测波长为254nm。结果:安替比林检测浓度在1.095～20mg·L-1范围内线性关系良好(r=0.9996);低、中、高3种浓度质控样品的日内RSD分别为5.03%、1.49%、1.53%,日间RSD分别为2.99%、1.01%、0.75%;平均方法回收率为97.19%～103.11%。结论:本方法简便可靠、专属性好、精密度高,可用于胎盘灌流液中安替比林浓度的测定。

OBJECTIVE： To establish the HPLC method for the concentration determination of antipyrine in placental perfusion. METHODS： The determination was performed on Waters Symmetry Ct8 column with mobile phase consisted of methanol-0.05 mol. L-1 phosphate buffer （pH6.5）（70：30） at the flow rate of 1 mL. min-1 using phenacetin as internal standard. The UV detection wavelength was at 254 mn. The concentration of antipyrine in placental perfusion was determined by liquid-liquid extraction pre-treatment. RESULTS： The linear range of antipyrine was 1.095~20 mg.L -1 （r=0.999 6）. The intra-day RSD of quality control were 5.03%, 1.49% and 1.53% ; the intar-day RSD of quality control were 2.99%, 1.01% and 0.75% for three concentrations of antipyrine. The average recoveries were 97.19% ~ 103.11%. CONCLUSION： The method is simple, reliable, specific and precise. It is suitable for determination of antipyrine in placental perfusion.