TSP-1、TGF-β和PPAR-γ在脑胶质瘤中的表达及其与微血管密度的关系

Expression of TSP-1,TGF-βand PPAR-γin glioma and relationships with microvascular density

目的探讨凝血酶敏感蛋白-1(TSP-1)、生长转化因子-β(TGF-β)和过氧化物酶体增殖物激活受体-γ(PPAR-γ)在脑胶质瘤中的表达及其与血管生成的关系。方法应用免疫组织化学法对62例胶质瘤组织中的TSP-1、TGF-β和PPAR-γ的表达及微血管密度(MVD)进行检测。结果 TSP-1、TGF-β和PPAR-γ在低级别胶质瘤中的阳性率分别为78.1%、43.7%和93.7%;在高级别胶质瘤中的阳性率分别为46.7%、93.3%和40.0%,PPAR-γ、TSP-1与TGF-β的表达均呈负相关(P<0.05)。低级别组MVD为26±5.2,高级别组MVD为41±7.3,二者之间的差异有统计学意义(P<0.05)。TSP-1、PPAR-γ与MVD呈负相关(P<0.05),TGF-β与MVD呈正相关(P<0.05))。结论 TSP-1、TGF-β、PPAR-γ与脑胶质瘤组织的MVD密切相关,可能参与了脑胶质瘤血管生成的调控。

Objective To explore expressions of thrombospondin-1 （TSP-1）, transforming growth factor-β（TGF-β）, and peroxisome proliferator activated receptor-y（PPAR-y） in glioma tissues and its correlations with tumor angiogenesis. Methods Expressions of TSP-1, TGF-β and PPAR-y and microvascular density （MVD） in 62 glioma cases of low and high grades were examined with SABC immunohistochemistry. Results The expression rates of TSP-1, TGF- β and PPAR-y in low-grade gliomas were 78.1%, 43.7% and 93.7% respectively, and expression rates in high-grade gliomas were 46.7%, 93.3% and 40.0% respectively, and expression of TSP-1 and PPAR-yboth had negative correlations with TGF-β（P 〈0.05）. It was found that the MVD of low-grade gliomas（26 ±5.2） was significantly lower than that of high-grade gliomas（ 4l ± 7.3 ） （P 〈 0.05 ）. TSP-1 and PPAR-yhad negative correlations with MVD（ P 〈 0.05 ）, and TGF-β had a positive correlation with MVD （ P 〈 0.05 ）. Conclusion The expression levels of TSP-1, TGF-β and PPAR-y are correlated with wlioma MVD and these factors may be involved in glioma angiogenesis.