PRRSV、PCV-2临床感染猪外周血中ACTH含量的变化

The content of ACTH in the serum of the pigs infected by PRRSV and/or PCV-2

采集61头有呼吸道疾病症状猪的病料,采用分子生物学方法进行猪繁殖与呼吸综合征病毒(PRRSV)、PRRSV变异株、猪圆环病毒2型(PCV-2)的检测,并采集血液分离血清,用放射性免疫法检测其外周血中促肾上腺皮质激素(ACTH)的含量.结果表明:PCV-2核酸阳性率为72.13%,PRRSV为39.34%,PRRSV变异株为22.95%;临床存在混合感染,特别是PRRSV与PCV-2的混合感染,感染率达16.39%;PRRSV、PRRSV变异株和PCV-2的感染均比对照组极显著地提高ACTH的含量,其中以PRRSV变异株与PRRSV的混合感染对ACTH的影响最大,ACTH含量达32.20 pg.mL-1.可见,PRRSV、PCV-2的感染促进了ACTH的分泌,增加猪的应激反应,从而降低病猪的免疫功能.

In order to determine the content of adrenocorticotrophic hormone（ACTH） in the serum of pigs which were infected by porcine reproductive and respiratory syndrone virus（PRRSV） and/or porcine circovirus type 2（PCV-2）,sixty-one specimens were collected from the pigs exhibited respiratory symptom around Fuzhou city,and PRRSV,PRRSV variant and/or PCV-2 were detected respectively.And the contents of ACTH in the serums were determined by radioimmunoassay.The results showed that the infection rate of PCV-2 was 72.13%,PRRSV was 39.34%,PRRSV variant was 22.95%.The mixed infection with PRRSV and PCV-2 was 16.39%.The contents of ACTH in the infected pigs were significantly increased compared with that of the control group（P0.01）,but the contents of ACTH among the pigs in different infection groups were not significant.The contents of ACTH of the pigs mixed infected with PRRSV and PRRSV variant were 32.20 pg·mL-1.Therefore,the contents of ACTH in the serum of the pigs infected by PRRSV and PCV-2 were elevated significantly,and the stress condition of the infected pigs was increased leading to the immune function reduced.