EDTA与多聚赖氨酸的铰链物抑制兔后发性白内障的实验研究

Experimental study of EDTA and poly-L-lysine hinge inhibiting after cataract in rabbit

目的:探讨EDTA与多聚赖氨酸的铰链物对兔后发性白内障的防治作用。方法:将20只新西兰白兔40眼随机分为A,B,C,D共4组,4组均行透明晶状体囊外摘除术。A组为对照组,术中灌注液为BSS;B,C,D组为治疗组,术中灌注液分别为浓度为10mg/L的EDTA、多聚赖氨酸、EDTA与多聚赖氨酸的铰链物的BSS溶液。2mo后行晶状体后囊膜切片,HE染色统计晶状体上皮细胞(LECs)的密度;并行免疫组织化学染色,用医学图象分析系统检测PCNA表达平均光密度(灰度值OD)。结果:经HE染色,后囊膜LECs密度B和D组较A和C组少,且D组的LECs密度小于B组,均有显著性差异(P<0.01);A和C组的LECs密度相差不大,无统计学差异(P>0.05)。免疫组织化学进行平均光密度测定,A和C组PCNA表达强阳性,B组呈部分阳性表达,D组阳性表达较B组更少。B和D组与A组、B组与D组均有显著性意义(P<0.01)。A组和C组无显著差异性(P>0.05)。结论:在活体兔眼中,EDTA、多聚赖氨酸与EDTA的铰链物均有抑制兔LECs增殖的作用,且EDTA与多聚赖氨酸的铰链物组对LECs的抑制作用优于EDTA组,多聚赖氨酸组对LECs的抑制作用不明显。

AIM： To explore the effect of EDTA and poly-L-lysine hinge on the rabbit after cataract （PCO） in the prevention and treatment role. METHODS： Forty eyes of twenty New Zealand white rabbits were randomly divided into A,B,C and D groups.The operations of extracapsular transparent lens extraction were performed on rabbit eyes in each group. Group A, the control group, was irrigated only with balanced salt solution （BSS） during operation. Group B,C,D, the treatment groups, were irrigated with diluted BSS of 10mg/L EDTA, poly-L-lysine, and EDTA and poly-L-lysine hinge. The opacity in posterior lens capsule was observed by routine examination after operation. Two months later,the densities of lens epithelial cells （ LECs） in the four groups were calculated through hematoxylin and eosin （HE） staining. The proliferating cell nuclear antigen（PCNA） and their expression were identified through immunohistochemical staining,and the average optical density （gray value OD） of PCNA expression was detected with medical image analysis system. RESULTS： After HE staining, the posterior capsule LECs density in group B,D was less than that in group A,C, and group D less than group B, the statistical analysis were significantly different （P0.01）;statistical analysis was no significant difference between group A and C （P0.05）. For the average optical density （gray） measured, PCNA expression in group A and group C were strongly positive, group B was partially positive, positive expression in group D was still less than that in group B. Statistical analysis of group B,D and group A, group B and D were significant difference （P0.01）. Group A and group C were no significant difference （P0.05）. CONCLUSION： In rabbits in vivo, EDTA, poly-L-lysine and EDTA hinge all have functions of inhibiting the proliferation of rabbit LECs, preventing and delaying the formation of PCO. Group D is superior to group B on inhibition of LECs, LECs inhibition of group C is not obvious.