脑不对称Balb/c小鼠血浆IL-1β、IL-6及皮质酮水平变化

The levels of Plasma IL-1, IL-6, corticosterone of Balb/c mice with lateralization

目的：研究脑不对称性对细胞因子和ＨＰＡ轴的影响。方法：用伸爪取食法将小鼠区分为左利、右利、双利动物，ＬＰＳ分别刺激２、４ｈ后取血浆用ＥＬＩＳＡ法检测ＩＬ－１β、ＩＬ－６、放免法测定血浆皮质酮含量。结果：左利组血浆ＩＬ－１β高于右利组，左利、右利组ＩＬ－１β均低于双利组。左利、右利组血浆皮质酮水平低于双利组。ＬＰＳ刺激后左利组ＩＬ－１β高于右利组，双利组亦高于右利组；左利、右利组血浆ＩＬ－６水平则均低于双利组。结论：脑不对称性对神经免疫内分泌网络有影响，左利者免疫紊乱发生率高可能与此有关。

Objective: To study the relationship between cytokines, HPA eds and lateralization. Methods: By using paw preference test,right-pawed,left-pawed, ambidextrous mice model were established. ELISA kits and radioimmunoassay were used for detection of Plasma levelsof cytokines and cortionterone. Results: In Untreated normal Balb/c mice, left-pawed mice had a higher IL-1 level than right-pawed nice (P< 0. 05), while IL-1 of ambidextrous was the highest among them; There was no difference observed between the the groups in plasma levelsof IL-6; As for Plasma levels of corticosterone in untreated mice, left-pawed and right-pawed mice were both lower than ambidextrous mice. InLPS treated mice, plasma levels of IL-1 were higher in left-pawed mice than in right-pawed and ambidextrous mice, and right-pawed mice waslower than ambidextrous; The plasma levels of IL-6 were lower in left-pawed and right-pawed mice than in ambidextrous(P < 0. 01 ). ThePlasma levels of corticosterone were almost the same among the three groups after the injection of LPS. Conclusion: Lateralization could influence cytokine levels and HPA axis in mice.