贵州矮杨梅叶总黄酮的提取工艺和测定方法

Extrating Crafts and Assay Method of Total Flavonoids in the Leaf of Myrica Nana in Guizhou

目的:建立矮杨梅叶总黄酮的提取工艺和含量测定方法。方法:利用L9(34)进行矮杨梅叶总黄酮提取影响因素即乙醇浓度、提取时间、料液比的优化组合;应用分光光度计考察其含量测定方法。结果:矮杨梅叶总黄酮的提取工艺为提取时间为1.5h,乙醇浓度为50%,材液比为1:35,提取率为4.88%;总黄酮提取液在0-60min内测定,线性关系良好(0.9996),仪器较为稳定,测定结果准确、重现性好,回收率高。结论:矮杨梅叶总黄酮的提取工艺可行、测定方法可信。这一研究结果对于杨梅属植物中总黄酮的提取和测定具有可操作性和推广应用价值。

Objective ： Establish the extrating crafts and assay method of total flavonoids in the leaf about M. nana. Method ： Optimized the factors of total flavonoids extrating crafts that ethanol concentration, extracting time and solid-liquid ratio by L9 （34） orthogonal table. Examined its method of determination by spectrophotometer. Result ; the extrating crafts as follows, ratio of material to solvent was 1：35, extracting time was 1.5h, eth- anol concentration was 50; ; extracting rate was 4. 88;;. In the course of 0-60min, the linear relationship of assay method was good（0. 9996）, the instrument was more stable, the result was accurate, the reproducibility and recovery was high. Conclusion ; The extracting technology was feasible and operable, it was important application value for the extraction of total flavonoids in Myrica plants.