内分泌因素影响牙槽骨修复的动物实验研究

Animal Experimental Study on Endocrine Factors Affecting Alveolar Repair

目的 :明确内分泌因素对牙槽骨修复过程中碱性磷酸酶 (ALP)和酸性磷酸酶 (ACP)活性的影响 ,并探讨牙槽骨吸收修复机制。方法 :2 4只狗的牙槽骨二壁骨袋模型 ,随机分成 4组 ,采用组织学检查、酶组织化学和全自动图像分析等技术定量分析去除卵巢、诱发实验性糖尿病及提高甲状腺素水平情况下 ,骨缺损修复过程中ALP和ACP活性变化。结果 :甲状腺素组除 6个月组ACP活性较高外 ,ALP与ACP活性均较对照组低 ,有统计学差异 (P <0 0 1) ;糖尿病组和卵巢切除组仅ALP降低有统计学差异 (P <0 0 5或P <0 0 1) ,酶的活性与骨形态结构变化相吻合。结论 :牙槽骨修复受全身内分泌因素的调控 ,且与其破骨细胞和成骨细胞的酶活性密切相关。

Objective: To investigate the endocrine effects on activities of alkaline phosphatase (ALP) and acid phosphatase (ACP) and to obtain the mechanism of the resorption and regeneration of the alveolar bone. Methods: 24 dog models with two side osseous infrabony pocket were divided into 4 groups randomly and were studied by histologic examination, enzyme cytochemistry and image analysis system. Results: The activities of ALP and ACP, except ACP in the sixth month significantly decreased in the group of thyroidism comparing with that of the control group (P<0 01), but only the activities of ALP significantly decreased in groups of ovariectomy and diabetes (P<0 05, P<0 01), and there was close relationship between enzyme activities and histopathologic variations. Conclusion: Alveolar bone repair was regulated by systemic endocrine factors and was associated with the activities of ALP and ACP osteoblasts of and osteoclasts.