表皮生长因子对重症胰腺炎肠外营养大鼠肠道细菌易位的影响

The Influence of EGF on Intestinal Bacterial Translocation in Rats with Severe Acute Pancreatitis during TPN.

探讨表皮生长因子（ＥＧＦ）对重症胰腺炎（ＳＡＰ）全肠外营养（ＴＰＮ）支持大鼠肠道细菌易位的影响。方法：４８只ＳＤ大鼠随机分为ＳＡＰ组、ＳＡＰ＋ＴＰＮ组及ＳＡＰ＋ＴＰＮ＋ＥＧＦ组。分别测定肠系膜淋巴结、肝脏及脾脏细菌易位率，利用图像分析仪对小肠粘膜进行检测，并测定回肠粘膜厌氧菌与需氧菌的比率。结果：ＳＡＰ＋ＴＰＮ＋ＥＧＦ组肠系膜淋巴结、肝脏及脾脏细菌易位率明显降低，小肠粘膜绒毛面积、高度、隐窝深度增加，回肠厌氧菌与需氧菌的比率显著提高。结论：ＥＧＦ具有保护重症胰腺炎全肠外营养大鼠肠粘膜机械屏障、生物屏障和降低肠道细菌易位率的作用。

To study the influence of EGF on intestinal bacterial translocation in rats with severe acute pancreatitis during TPN. Methods: Forty-eight SD rats were randomly divided into SAP, SAP+TPN and SAP+TPN+EGF groups. The bacterial translocation in mesenteric lymph nodes, liver and spleen was examed. Image processing and analysis system was used to study the histology of intestinal mucosa, and ratio of Bifidobacterium/E. coli in intestine was examed. Results: The bacterial translocation in mesenteric lymph nods, liver and spleen decreased significantly, and villus height, villus area, crypt depth and Bifidobacterium/E. coli ratio were increased in SAP+TPN+EGF group. Conclusions: EGF can protect the intestinal cellular and biological barrier, and decrease the bacterial translocation in rats with severe acute pancreatitis during TPN.