云南瑞丽HIV-1感染长期存活者的病毒分离及其生物学特性的研究

Viral Isolation from Long - term Survivors with HIV - 1 Infections and Analysis of the Viral Biological Features in Southwest of China

目的 研究长期不进展者的病毒生物学特征及其与疾病发展的关系。方法 用感染者和正常人外周血单核细胞(PBMC)共培养的方法分离病毒,终点以共培养上清液中的p24抗原作为病毒生长的评价指标。结果 (1)从15例长期不进展者分离到4株病毒,占26.7％。同时取该地区HIV-1感染进展者血样20份,分离到14株病毒,阳性率为70.0％;(2)病毒分离率影响因素的试验结果表明,病毒的分离率与CD_4细胞数有明显关系,CD_4细胞数越高,分离率越低。反之,CD_4细胞数越低,病毒分离率越高。此外,去除CD_8细胞后可以显著提高病毒的分离率;(3)病毒生物学特性观察发现长期不进展者分离株均属生长缓慢、低滴度、非致细胞融合型(NSI型),在T细胞不能生长的病毒;(4)大部分进展者分离株与长期不进展者分离株在生物学特性方面没有显示区别。结论 长期不进展者与大部分进展者的病毒生物学特征没有明显区别,因此,推测病毒学因素可能不是决定病程进展的最主要因素。影响疾病进展的其它因素,如免疫因素等尚需进一步研究。

To study relationship of the HIV from the disease progressors and one isolated from long - term survivors (LTS). Method HIV- 1 isolation was done by co-culture with peripheral blood mononuclear cells (PBMC) from the positive and negetive individuals. Viral antigen p24 of the cultural supernatants was used as a maker of the viral replication. Results (1)4 HIV- 1 strains were isolated from 15 LTS (26.7% ), and 14 ones from 20 HIV- 1 infected progressors in the same area (70% ); (2) The rate of viral isolation was obviously related to the numbers of CD4 + cells, the lower of CD4 cells, the higher the rate of the viral isolation. The viral isolation was also obviously increased by exclusion of CD8 + cells in co-culture; (3) The viral strains isolated from LTS grew slowly with a low titer in vitro without syncytia and could not replicate in T cell lines. Sequence analysis also shown that the isolated strains were non-synctyium (NSI) type; (4)The biological features of the most strains from the progressors were silimar with those from LTS. Conclusion No obvious difference in biological features is found between the LTS and the most of progressor strains. Therefore, It is possible that viro-logical factor is not a key factor affecting the disease progression.