白介素8在冠心病急性心肌梗死患者中的变化及临床意义

The change and role of serum interleukin-8 in the patients with acute myocardial infarction

目的 :本实验通过对冠心病急性心肌梗死 (AMI)患者及AMI溶栓治疗前后白细胞介素 8(IL 8)变化的研究 ,为AMI的早期诊断及溶栓治疗提供可供参考的依据。方法 :AMI患者 ,依据治疗情况分为 :(1)溶栓组 ,于溶栓即刻、溶栓后 2、 4、 6、 8、 10、 12h测定血清中IL 8浓度 ,并依据溶栓结果分为 :溶栓再通组 (A组 )、溶栓未通组 (B组 )。 (2 )未溶栓组 ,于症状出现后 2、 4、 6、 8、 10、 12、 14h测定血清中IL 8浓度。IL 8浓度应用酶联免疫吸附(ELISA)法测定。同时测定血清心肌酶CK MB的浓度。结果 :(1)IL 8在AMI患者症状出现后4 47± 1 5 0h升高 ,水平明显高于正常对照组 ,时间早于CK MB升高的时间 (P <0 0 1) ,并可在 9 6 9± 3 5 4h时达到高峰 ;(2 )A组IL 8浓度在溶栓后 4、 6、 8h显著高于B组 (P <0 0 1、P<0 0 1、P <0 0 5 ) ,B组IL 8浓度与未溶栓组之间无显著区别。 (3)正常对照组IL 8浓度 <10pg/ml。结论 :(1)AMI患者血清中很早即可测得IL 8浓度 ,水平明显高于正常对照组 ,时间早于CK MB升高的时间 ,故认为IL 8可作为AMI早期诊断的参考指标之一 ;(2 )溶栓再通组可于溶栓后 4、 6、 8h检测到明显升高的IL 8浓度 ,故认为IL

Objective:To study the change and role of serum interleukin 8(IL 8) in patients with acute myocardial infarction (AMI) who received and did not receive thrombolytic treatment might be helpful to diagnose and to treat patients with AMI.Methods:(1)Thrombolytic group.In patients who received thrombolytic treatment,serum IL 8 was measured before and 2、4、6、8、10and 12h after the treatment.This group was subdivided according to the results of thrombolytic treatment:group A,patients who developed reperfusion and group B,patients who did not receive reperfusion.(2)Nonthrombolytic group,In patients who did not receive thrombolytic treatment,serum IL 8 was measured at 2、4、6、8、10、12 and 14h after the onset of complaints.Serum IL 8 was measured by ELISA,and CK MB was measured at the same time.Results:(1)Serum IL 8 concentration would be elevated at 4 47±1 50h after the onset of complaints in patients with AMI,earlier than that with CK MB(P<0 01),IL 8 concentration is markedly highly than that of control group,and the maximum value would be obtained at 9 69±3 54h.(2)Serum 12 8 concentration of group A was markedly higher than that of group B at 4,6,and 8h after thrombolytic treatment(P<0 01,P<0 01,P<0 05).IL 8 concentration of group B was not different from the group which did not receive thrombolytic treatment.(3)Serum IL 8 concentration of normal control group was<10pg/ml.Conclusions:(1)Serum IL 8 concentration would be elevated in the early phase in AMI,earlier than CK MB,IL 8 concentration is markedly higher that of the control.IL 8 may be thought to be one of the reference markers of the diagnosis in early phase of AMI.(2)A markedly high concentration of IL 8 would be measured at 4,6 and 8h after thrombolytic treatment in patients who developed reperfusion.So IL 8 maybe one of the reference markers to determine who patients who receive thrombolytic treatment would develop reperfusion.