大蒜预防口腔癌变过程中上皮细胞内微量元素的分析

Study on trace elements in epithelial cells during oral carcinogenesis prevented by garlic

将 62只Wistar大鼠均分为二组 ,于实验组 (3 1只 )大鼠硬腭后部涂大蒜液 ,对照组涂等量蒸馏水 ;随后再分别涂 0 5 % 4 硝基喹啉氧化物 ,每周三次 ,共 19周 ,应用电子探针显微分析仪观测不同时期二组动物腭粘膜上皮或癌细胞内微量元素的变化。结果示 ,实验组上皮细胞核内、外Cu含量明显降低 ,早期细胞核内、外Se含量及晚期Zn水平升高 ;各期元素间比值亦有不同程度改变。研究提示 ,大蒜预防口腔癌变与其调节细胞核内、外Cu ,Zn及Se等元素含量有关。

Sixty two Wistar rats were divided randomly into two groups, thirty one for each group. The posterior hard palatal mucosae of all animals were painted thrice weekly with 0.5% 4 nitroquinoline 1 oxide(dissolved in dimethyl sulfoxide). Before that, the garlic injection solution and the distilled water were painted at the same place of the experimental and control group animals, respectively. All animals were killed in turn from the beginning of the experiment at random at the 10th, 13th, and 19th week. Then, trace elements of intranuclei and cytoplasm of epithelial cells or cacer cells at the mentioned weeks were surveyed by electron probe micro analysis. The results were that garlic decreased the levels of intranuclear and cytoplasm copper(P<0.05); the levels of intranuclear and cytoplasm selenium at the 10th week and the 13th week(P<0.05) and those of zinc at the 19th week (P<0.01) increased. So, garlic inhibits oral carcinogenesis by changing concentrations of intranuclear and cytoplasm trace elements that is copper, zinc, selenium, and the ratio of the three elements.