鹿茸多肽对实验性膝骨性关节炎关节软骨中糖胺多糖和Ⅱ型胶原水平的影响

Effects of Pilose antler polypeptide on the glycosaminoglycan and type Ⅱ collagen in experimental knee osteoarthritis

目的:检测膝骨性关节炎关节软骨中糖胺多糖和Ⅱ型胶原的水平及鹿茸多肽对其影响。方法:6月龄新西兰大白兔64只,随机分为正常组(8只)和模型组(56只)。模型组采用Hulth法造成膝骨性关节炎模型。造模成功后再将模型组随机分为鹿茸多肽组(24只)和对照组(24只),鹿茸多肽组给予鹿茸多肽稀释液0.5ml,每2日1次关节腔注射;对照组给予0.5ml生理盐水,每2日1次关节腔注射。分别于干预后第7、15、30天分3批取材,甲苯胺蓝染色观察两组关节软骨中糖胺多糖含量,免疫组织化学法观察软骨基质中Ⅱ型胶原的表达。结果:随着干预时间的延长,鹿茸多肽组和对照组软骨基质中糖胺多糖和Ⅱ型胶原表达量逐渐减少。鹿茸多肽组药物干预后第7、15、30天,Ⅱ型胶原阳性面积积分光密度值分别为(312.06±14.12)、(273.31±12.42)和(248.34±10.41),组间差异有统计学意义(P<0.05);对照组干预后第7、15、30天,Ⅱ型胶原阳性面积积分光密度值分别为(253.47±15.53)、(215.67±9.72)和(160.01±13.23),组间差异有统计学意义(P<0.05)。相同时间段内,鹿茸多肽组Ⅱ型胶原阳性面积积分光密度值高于对照组,组间差异有统计学意义。结论:鹿茸多肽通过阻止膝骨性关节炎关节软骨基质中糖胺多糖和Ⅱ型胶原含量的减少,一定程度上延缓关节软骨的退变。

Objective：To observe the influence of Pilose antler polypeptide on the glycosaminoglycan and typeⅡcollagen in the articular cartilage in experimental knee osteoarthritis. Methods：Totally 64 New Zealand white rabbits of 6 months old were randomly divided into 2 groups：normal group（n=8）and model group （n=56）. Model group was surgically induced into osteoarthritis model by method of Hulth. After successful modeling,the rabbits of model group were further divided into 2 groups：Pilose antler polypeptide -treatment group and control group,24 rabbits in each group. Pilose antler polypeptide-treatment group received 0.5 ml intra-articular injection of Pilose antler polypeptide dilution liquid once in per 2 days for 30 days,while control group received 0.5 ml intra-articular injection of physiological saline. On days 7,15 and 30 after intervention,articular cartilage samples were collected respectively. The content of glycosaminoglycan in articular cartilage was observed by toluidine blue staining and the expression of typeⅡcollagen in cartilage matrix was detected by immunohistochemical staining. Results：Along with the prolonging of time,the content of glycosaminoglycan and typeⅡcollagen in cartilage matrix of the Pilose antler polypeptide-treatment group and control group decreased gradually. On days 7,15 and 30 after intervention,integrated optical density of the typeⅡcollagen positive area in cartilage matrix of the Pilose antler polypeptide-treatment group were （312.06±14.12）,（273.31±12.42） and （248.34±10.41）,which had statistically significant differences. Integrated optical density of the typeⅡ collagen positive area in cartilage matrix of the control group were （253.47±15.53）,（215.67±9.72） and （160.01±13.23）,which had statistically significant differences. At the same period,integrated optical density of the typeⅡcollagen positive area in cartilage matrix of the Pilose antler polypeptide-treatment group was higher than that of control group,which had statistically significant difference. Conclusion：Pilose antler polypeptide can inhibit reduction of the glycosaminoglycan and typeⅡcollagen in cartilage matrix and delay the degeneration of articular cartilage.