高效液相色谱法测定人体血浆中鲁拉西酮的含量

HPLC determination of lurasidone in human plasma

目的:建立高效液相色谱法测定人体血浆中鲁拉西酮的含量。方法:色谱柱为美国Diamonsil(250 mm×4.6 mm,5μm)C18反相柱;流动相为乙腈-甲醇-缓冲液(35∶15∶50),流速为1.2 mL.min-1;检测波长256 nm。结果:鲁拉西酮血浆浓度在0.018～2.304μg.mL-1范围内线性关系良好,平均相对回收率为97.08%,平均日间精密度为4.58%。结论:本方法具有快速、简便、灵敏度高、重复性好等优点,可以用于鲁拉西酮临床研究和不良反应监测。

OBJECTIVE To establish the determination of lurasidone in plasma by HPLC. METHODS Column was Diamonsil C18 （250 mm ×4.6mm,5μm） ; Mobile phase was acetonitrile： MeOH ： Buffer （35 ： 15 ： 50）, the flow rate was l. 2 mL. min^- 1 UV wavelength was 256 nrru RESULTS Within a concentration range of 0. 018 - 2. 304μg. mL^-1 , the standard curve of lurasi- done had a good linearity. The average relative recovery was 97. 08%, the average daytime precision was 4. 58 %. CONCLUSION It appears to be a rapid, convenient, sensitive and reproducible method for clinical research and adverse reaction monitor of lurasidone.