高效液相色谱-二极管阵列检测法同时测定双黄连片剂中的12种化合物

Simultaneous determination of twelve chemical components in Shuang-huang-lian tablet by HPLC-DAD

目的:建立同时测定双黄连片中12种化学成分(绿原酸、咖啡酸、芦丁、金丝桃苷、连翘酯苷、野黄芩苷、黄芩苷、连翘苷、木犀草素、芹菜素、黄芩素、汉黄芩素)的高效液相色谱方法。方法:采用高效液相色谱法,色谱柱为Aglient Zorbax SB-C18(4.6 mm×250 mm,5μm);流动相为乙腈-0.1%甲酸水溶液,梯度洗脱,流速为1.0mL.min-1;检测波长为320 nm(0～50min)和278 nm(50.1～105 min),柱温为35℃。结果:12种化学成分均达到基线分离,各成分均有较宽的线性范围和良好的线性关系(r>0.999 2)。回收率范围在95.5%～102.3%,RSD为1.0%～1.9%。结论:本方法准确、可靠,重复性较好,可作为双黄连片的质量控制方法之一。

OBJECTIVE To establish an HPLC method for determination of the content of twelve chemical components in the Shuang-huang-lian （SHL） tablet, namely chlorogenic acid, caffeic acid, rutin, hyperoside, forsythoside, scutellarin, baicalin, forsythin, luteoloside,apigenin, baicalein, wogonin. METHODS The chromatographic separation was performed on an Aglient Zorbax SB-C18 （4. 6 mm×250mm,5μm）column with a gradient elution program using a mixture of acetonitrile and 0. 1%for- mic acid as mobile phase. Flow rate was 1.0 mLomin 1. The （detection wavelength was set at 320 nm （0 - 50 min） and 278 nm （50. 1 - 105 min）. Column temperature was 35 ℃. RESULTS The twelve analyses showed good regression （r〉0. 999 2） with- in test ranges and the recovery of the method was in the range of 95. 5% - 102. 3%. CONCLUSION The method was accurate, reliable, repeatable, and could be readily utilized as a quality control method for SHL tablet.