摘要
目的:初步探讨对于直接或间接引起的肺内感染,炎症反应产生的白介素(IL)-22能否刺激肺泡Ⅱ型上皮细胞,并且诱导其生成、释放2型人β防御素(humanβ-defensin-2,HBD-2)发挥抗感染作用。方法:流式细胞术检测肺泡Ⅱ型上皮细胞细胞膜表面IL-22R表达水平。重组人IL-22(20 ng/ml)刺激肺泡Ⅱ型上皮细胞,RT-PCR检测不同时间(0、2、4、8、16、24 h)HBD-2表达情况。不同浓度的重组人IL-22(0、4、20、100、500 ng/ml)刺激肺泡Ⅱ型上皮细胞,RT-PCR检测HBD-2表达情况。结果:肺泡Ⅱ型上皮细胞膜表面IL-22R表达呈强阳性,阳性率达(99.90±0.13)%。比较6个时间点HBD-2表达差异,发现HBD-2表达具有时间依赖性。比较不同浓度IL-22处理下HBD-2表达差异,IL-22 100 ng/ml为最佳干预浓度,且HBD-2表达与IL-22浓度具有依赖关系。结论:IL-22能显著诱导肺泡上皮细胞生成并释放HBD-2,且具有时间和剂量依赖性。
Objective:To investigate whether human alveolar type Ⅱ epithelial cells express IL-22R and whether it generates hu- man -defensin-2 (HBD-2) under the stimulation with IL-22. Methods: First,flow cytometry (FCM) detected the expression of IL- 22R on alveolar type Ⅱ epithelial cells;second,cells were stimulated with recombinant human IL-22 (rhIL-22) for 0,2,4,8,16,24 hours,separately,and then RT-PCR tested the expression of HBD-2;third,the cells were stimulated with different concentration (0,4,20,100,500 ng/ml) of IL-22 for optimal dose. Results: The membranes of alveolar type Ⅱ epithelial cells highly expressed IL-22R and the positive rate is (99.90 ± 0.13)%;24 hours is an effective time for HBD-2 expression with IL-22 treatment,and the optimal concentration is 100 ng/ml. Conclusion: The alveolar type Ⅱ epithelial cells highly express IL-22R, and the interaction of IL-22 and IL-22R induces the HBD-2 gene expression in alveolar epithelium in a timeand concentration-dependent manner.
出处
《南京医科大学学报(自然科学版)》
CAS
CSCD
北大核心
2012年第2期199-203,共5页
Journal of Nanjing Medical University(Natural Sciences)